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- W2065325873 abstract "Staining with the fluorochrome DAPI was investigated as an alternative to Feulgen staining for measuring DNA contents of zoospore nuclei. Five isolates of P. infestans with variable DNA content gave the same rank order for both methods. DNA contents of hyphal nuclei had similar mean values to those of zoospore nuclei indicating that zoospore nuclei are unreplicated and not in synthesis phase. Both A1 and A2 isolates from selected countries showed a range of DNA contents from a basic 2C level, presumed diploid, through intermediate quantities to 4C, presumed tetraploid. A similar variation was seen in freshly acquired British field isolates. Evidence was produced that one isolate having a bimodal distribution of DNA contents was a heterokaryon with unreplicated nuclei of 2C and 4C values. Chromosome counts on DAPI stained meiotic metaphases of gametangia from 2C × 2C and 4C × 4C matings indicated that 2C isolates had n = 8–12 and 4C isolates had n = 14–20. Thus isolates with approximately 4C values were shown to be tetraploid." @default.
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- W2065325873 date "1991-05-01" @default.
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- W2065325873 title "Variation in DNA content of nuclei of Phytophthora infestans as measured by a microfluorimetric method using the fluorochrome DAPI" @default.
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- W2065325873 doi "https://doi.org/10.1016/s0953-7562(09)80074-9" @default.
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