FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2065346543> ?p ?o ?g. }

• W2065346543 endingPage "e47734" @default.
• W2065346543 startingPage "e47734" @default.
• W2065346543 abstract "Loss of epithelial polarity is described as a hallmark of epithelial cancer. To determine the role of Hugl1 and Hugl2 expression in the breast, we investigated their localization in human mammary duct tissue and the effects of expression modulation in normal and cancer cell lines on polarity, proliferation and differentiation. Expression of Hugl1 and Hugl2 was silenced in both MCF10A cells and Human Mammary Epithelial Cells and cell lines were grown in 2-D on plastic and in 3-D in Matrigel to form acini. Cells in monolayer were compared for proliferative and phenotypic changes while acini were examined for differences in size, ability to form a hollow lumen, nuclear size and shape, and localization of key domain-specific proteins as a measure of polarity. We detected overlapping but distinct localization of Hugl1 and Hugl2 in the human mammary gland, with Hugl1 expressed in both luminal and myoepithelium and Hugl2 largely restricted to myoepithelium. On a plastic surface, loss of Hugl1 or Hugl2 in normal epithelium induced a mesenchymal phenotype, and these cells formed large cellular masses when grown in Matrigel. In addition, loss of Hugl1 or Hugl2 expression in MCF10A cells resulted in increased proliferation on Matrigel, while gain of Hugl1 expression in tumor cells suppressed proliferation. Loss of polarity was also observed with knockdown of either Hugl1 or Hugl2, with cells growing in Matrigel appearing as a multilayered epithelium, with randomly oriented Golgi and multiple enlarged nuclei. Furthermore, Hugl1 knock down resulted in a loss of membrane identity and the development of cellular asymmetries in Human Mammary Epithelial Cells. Overall, these data demonstrate an essential role for both Hugl1 and Hugl2 in the maintenance of breast epithelial polarity and differentiated cell morphology, as well as growth control." @default.
• W2065346543 created "2016-06-24" @default.
• W2065346543 creator A5020891622 @default.
• W2065346543 creator A5028304724 @default.
• W2065346543 creator A5058984558 @default.
• W2065346543 creator A5088754139 @default.
• W2065346543 date "2012-10-23" @default.
• W2065346543 modified "2023-10-16" @default.
• W2065346543 title "Hugl1 and Hugl2 in Mammary Epithelial Cells: Polarity, Proliferation, and Differentiation" @default.
• W2065346543 cites W1521774859 @default.
• W2065346543 cites W1566467768 @default.
• W2065346543 cites W1966037423 @default.
• W2065346543 cites W1966639607 @default.
• W2065346543 cites W1976577398 @default.
• W2065346543 cites W1976878917 @default.
• W2065346543 cites W1980039397 @default.
• W2065346543 cites W1981265221 @default.
• W2065346543 cites W1983350492 @default.
• W2065346543 cites W1985262373 @default.
• W2065346543 cites W1990490057 @default.
• W2065346543 cites W1994404140 @default.
• W2065346543 cites W2002602524 @default.
• W2065346543 cites W2004434166 @default.
• W2065346543 cites W2005993913 @default.
• W2065346543 cites W2008506771 @default.
• W2065346543 cites W2010399610 @default.
• W2065346543 cites W2015128080 @default.
• W2065346543 cites W2018574900 @default.
• W2065346543 cites W2019018578 @default.
• W2065346543 cites W2024166544 @default.
• W2065346543 cites W2024585236 @default.
• W2065346543 cites W2034653929 @default.
• W2065346543 cites W2038893299 @default.
• W2065346543 cites W2049180247 @default.
• W2065346543 cites W2062975096 @default.
• W2065346543 cites W2074217810 @default.
• W2065346543 cites W2080042207 @default.
• W2065346543 cites W2083042692 @default.
• W2065346543 cites W2086442201 @default.
• W2065346543 cites W2098003895 @default.
• W2065346543 cites W2103078867 @default.
• W2065346543 cites W2109319525 @default.
• W2065346543 cites W2115583685 @default.
• W2065346543 cites W2128680169 @default.
• W2065346543 cites W2131519618 @default.
• W2065346543 cites W2141760038 @default.
• W2065346543 cites W2146071146 @default.
• W2065346543 cites W2153185706 @default.
• W2065346543 cites W2157488888 @default.
• W2065346543 cites W216085092 @default.
• W2065346543 cites W2162412654 @default.
• W2065346543 cites W2185708691 @default.
• W2065346543 cites W83974255 @default.
• W2065346543 doi "https://doi.org/10.1371/journal.pone.0047734" @default.
• W2065346543 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3479147" @default.
• W2065346543 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23110097" @default.
• W2065346543 hasPublicationYear "2012" @default.
• W2065346543 type Work @default.
• W2065346543 sameAs 2065346543 @default.
• W2065346543 citedByCount "34" @default.
• W2065346543 countsByYear W20653465432013 @default.
• W2065346543 countsByYear W20653465432014 @default.
• W2065346543 countsByYear W20653465432015 @default.
• W2065346543 countsByYear W20653465432016 @default.
• W2065346543 countsByYear W20653465432017 @default.
• W2065346543 countsByYear W20653465432018 @default.
• W2065346543 countsByYear W20653465432019 @default.
• W2065346543 countsByYear W20653465432020 @default.
• W2065346543 countsByYear W20653465432021 @default.
• W2065346543 countsByYear W20653465432022 @default.
• W2065346543 countsByYear W20653465432023 @default.
• W2065346543 crossrefType "journal-article" @default.
• W2065346543 hasAuthorship W2065346543A5020891622 @default.
• W2065346543 hasAuthorship W2065346543A5028304724 @default.
• W2065346543 hasAuthorship W2065346543A5058984558 @default.
• W2065346543 hasAuthorship W2065346543A5088754139 @default.
• W2065346543 hasBestOaLocation W20653465431 @default.
• W2065346543 hasConcept C104317684 @default.
• W2065346543 hasConcept C140884156 @default.
• W2065346543 hasConcept C148738053 @default.
• W2065346543 hasConcept C1491633281 @default.
• W2065346543 hasConcept C203014093 @default.
• W2065346543 hasConcept C204232928 @default.
• W2065346543 hasConcept C2778608917 @default.
• W2065346543 hasConcept C39587587 @default.
• W2065346543 hasConcept C49353375 @default.
• W2065346543 hasConcept C529295009 @default.
• W2065346543 hasConcept C54355233 @default.
• W2065346543 hasConcept C55493867 @default.
• W2065346543 hasConcept C62112901 @default.
• W2065346543 hasConcept C81885089 @default.
• W2065346543 hasConcept C86803240 @default.
• W2065346543 hasConcept C95444343 @default.
• W2065346543 hasConceptScore W2065346543C104317684 @default.
• W2065346543 hasConceptScore W2065346543C140884156 @default.
• W2065346543 hasConceptScore W2065346543C148738053 @default.
• W2065346543 hasConceptScore W2065346543C1491633281 @default.
• W2065346543 hasConceptScore W2065346543C203014093 @default.

• next