FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2065364802> ?p ?o ?g. }

• W2065364802 endingPage "1830" @default.
• W2065364802 startingPage "1822" @default.
• W2065364802 abstract "On the basis of their versatile structure and chemistry as well as tunable mechanical properties, polymer brushes are well-suited as supports for enzyme immobilization. However, a robust surface design is hindered by an inadequate understanding of the impact on activity from the coupling motif and enzyme distribution within the brush. Herein, horseradish peroxidase C (HRP C, 44 kDa), chosen as a model enzyme, was immobilized covalently through its lysine residues on a N-hydroxysuccinimidyl carbonate-activated poly(2-hydroxyethyl methacrylate) (PHEMA) brush grafted chemically onto a flat impenetrable surface. Up to a monolayer coverage of HRP C is achieved, where most of the HRP C resides at or near the brush-air interface. Molecular modeling shows that lysines 232 and 241 are the most probable binding sites, leading to an orientation of the immobilized HRP C that does not block the active pocket of the enzyme. Michaelis-Menten kinetics of the immobilized HRP C indicated little change in the K(m) (Michaelis constant) but a large decrease in the V(max) (maximum substrate conversion rate) and a correspondingly large decrease in the k(cat) (overall catalytic rate). This indicates a loss in the percentage of active enzymes. Given the relatively ideal geometry of the HRPC-PHEMA brush, the loss of activity is most likely due to structural changes in the enzyme arising from either secondary constraints imposed by the connectivity of the N-hydroxysuccinimidyl carbonate linking moiety or nonspecific interactions between HRP C and DSC-PHEMA. Therefore, a general enzyme-brush coupling motif must optimize reactive group density to balance binding with neutrality of surroundings." @default.
• W2065364802 created "2016-06-24" @default.
• W2065364802 creator A5002684335 @default.
• W2065364802 creator A5017951751 @default.
• W2065364802 creator A5022835935 @default.
• W2065364802 creator A5033348185 @default.
• W2065364802 creator A5036590305 @default.
• W2065364802 creator A5040711609 @default.
• W2065364802 creator A5048884097 @default.
• W2065364802 creator A5054299179 @default.
• W2065364802 date "2011-04-15" @default.
• W2065364802 modified "2023-09-27" @default.
• W2065364802 title "Poly(2-hydroxyethyl methacrylate) for Enzyme Immobilization: Impact on Activity and Stability of Horseradish Peroxidase" @default.
• W2065364802 cites W1571455001 @default.
• W2065364802 cites W1938981932 @default.
• W2065364802 cites W1973076220 @default.
• W2065364802 cites W1973847610 @default.
• W2065364802 cites W1976558559 @default.
• W2065364802 cites W1978177736 @default.
• W2065364802 cites W1980357833 @default.
• W2065364802 cites W1981252140 @default.
• W2065364802 cites W1988956914 @default.
• W2065364802 cites W1989110750 @default.
• W2065364802 cites W1993857085 @default.
• W2065364802 cites W1996294361 @default.
• W2065364802 cites W2000694345 @default.
• W2065364802 cites W2008303284 @default.
• W2065364802 cites W2015694411 @default.
• W2065364802 cites W2018525822 @default.
• W2065364802 cites W2024643348 @default.
• W2065364802 cites W2033413023 @default.
• W2065364802 cites W2042548216 @default.
• W2065364802 cites W2043451183 @default.
• W2065364802 cites W2051734899 @default.
• W2065364802 cites W2059636489 @default.
• W2065364802 cites W2074450344 @default.
• W2065364802 cites W2078139254 @default.
• W2065364802 cites W2082111034 @default.
• W2065364802 cites W2085803356 @default.
• W2065364802 cites W2086189101 @default.
• W2065364802 cites W2112659494 @default.
• W2065364802 cites W2114225171 @default.
• W2065364802 cites W2139974724 @default.
• W2065364802 cites W2140945887 @default.
• W2065364802 cites W2149996560 @default.
• W2065364802 cites W2154998149 @default.
• W2065364802 cites W2158534713 @default.
• W2065364802 cites W2165898505 @default.
• W2065364802 cites W4234821530 @default.
• W2065364802 cites W4376595447 @default.
• W2065364802 cites W3142063049 @default.
• W2065364802 doi "https://doi.org/10.1021/bm200173y" @default.
• W2065364802 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21438540" @default.
• W2065364802 hasPublicationYear "2011" @default.
• W2065364802 type Work @default.
• W2065364802 sameAs 2065364802 @default.
• W2065364802 citedByCount "52" @default.
• W2065364802 countsByYear W20653648022012 @default.
• W2065364802 countsByYear W20653648022013 @default.
• W2065364802 countsByYear W20653648022014 @default.
• W2065364802 countsByYear W20653648022015 @default.
• W2065364802 countsByYear W20653648022016 @default.
• W2065364802 countsByYear W20653648022017 @default.
• W2065364802 countsByYear W20653648022018 @default.
• W2065364802 countsByYear W20653648022019 @default.
• W2065364802 countsByYear W20653648022020 @default.
• W2065364802 countsByYear W20653648022021 @default.
• W2065364802 countsByYear W20653648022022 @default.
• W2065364802 crossrefType "journal-article" @default.
• W2065364802 hasAuthorship W2065364802A5002684335 @default.
• W2065364802 hasAuthorship W2065364802A5017951751 @default.
• W2065364802 hasAuthorship W2065364802A5022835935 @default.
• W2065364802 hasAuthorship W2065364802A5033348185 @default.
• W2065364802 hasAuthorship W2065364802A5036590305 @default.
• W2065364802 hasAuthorship W2065364802A5040711609 @default.
• W2065364802 hasAuthorship W2065364802A5048884097 @default.
• W2065364802 hasAuthorship W2065364802A5054299179 @default.
• W2065364802 hasConcept C111368507 @default.
• W2065364802 hasConcept C118792377 @default.
• W2065364802 hasConcept C121332964 @default.
• W2065364802 hasConcept C127313418 @default.
• W2065364802 hasConcept C132050475 @default.
• W2065364802 hasConcept C148898269 @default.
• W2065364802 hasConcept C161790260 @default.
• W2065364802 hasConcept C162008176 @default.
• W2065364802 hasConcept C166940927 @default.
• W2065364802 hasConcept C178790620 @default.
• W2065364802 hasConcept C180577832 @default.
• W2065364802 hasConcept C181199279 @default.
• W2065364802 hasConcept C185592680 @default.
• W2065364802 hasConcept C188027245 @default.
• W2065364802 hasConcept C21951064 @default.
• W2065364802 hasConcept C2776568683 @default.
• W2065364802 hasConcept C2777289219 @default.
• W2065364802 hasConcept C2777676563 @default.
• W2065364802 hasConcept C2781018886 @default.
• W2065364802 hasConcept C28212737 @default.
• W2065364802 hasConcept C33633552 @default.

• next