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- W2065368130 abstract "Abstract A method for the separation of C-24 epimers of brassinosteroids by reversed-phase liquid chromatography (LC) is described. The steroids, brassinolide, 24-epibrassinolide, castasterone and 24-epicastasterone, were derivatized quantitatively into bis-m-aminophenylboronates through both the 2α,3α- and 22,23-dihydroxy groups by treatment with m-aminophenylboronic acid in the presence of pyridine in acetonitrile. Subsequent resolution into each epimer was attained by LC on an ODS column using acetonitrile - 1% (v/v) acetic acid (75 + 25) containing 20 mM 18-crown-6 as the mobile phase. The boronates of C-24 epimeric pairs of brassinosteroids were monitored by fluorimetric detection using a postcolumn reaction system with o-phthalaldehyde - cyanide ion, the limit of detection being 40 pg for brassinolide." @default.
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- W2065368130 date "1992-01-01" @default.
- W2065368130 modified "2023-09-27" @default.
- W2065368130 title "Effective separation of C-24 epimeric brassinosteroids by liquid chromatography" @default.
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- W2065368130 doi "https://doi.org/10.1016/0003-2670(92)85361-9" @default.
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