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- W2065371036 abstract "Adult male rats weighing about 200 g were randomized into three dietary groups. One group was fed a high level 20.55%, the second group a medium level 16.44% and the third group a low level 12.33% of casein protein. Water was allowed ad libitum. Urinary metabolites including ammonia, urea (enzymatic methods) and total nitrogen (chemiluminescence) were determined and hence the nitrogen balance data were computed. Free amino acid patterns were examined in plasma and tissue (liver, muscle and kidney) by a high performance liquid chromatographic method. Urinary metabolites were significantly depressed as the level of dietary protein decreased. Plasma free amino acids showed a decreasing concentration of the essential amino acids and a concomitant increase of those of the non essential amino acids. In the liver, the total concentrations of free amino acids were higher at low levels of protein intake, while the total concentrations of the free amino acids were essentially similar in the muscle regardless of the level of protein intake. Animals receiving low protein diet had greater conservation and presumably better utilization of nitrogen, perhaps explained in terms of general adaptation." @default.
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- W2065371036 date "1987-03-01" @default.
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- W2065371036 title "Effect of dietary protein restriction on urinary nitrogen loss and tissue free amino acid patterns in mature rats" @default.
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- W2065371036 doi "https://doi.org/10.1016/s0271-5317(87)80018-0" @default.
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