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- W2065384582 abstract "Frequency-domain fluorometry relies on the measurement of the phase and amplitudes of the Fourier components of the time-dependent fluorescence signal. Experimental results that show that a conventional photomultiplier is subject to intensity-dependent phase shifts are presented. The measurements indicate that this is a problem well below the maximum linear current of the photomultiplier response. These results have important implications in frequency-domain fluorescence anisotropy experiments, in which the parallel and the perpendicular components of the emission intensity are inherently different from one another: a phase shift can be introduced by the photomultiplier." @default.
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- W2065384582 date "1993-02-20" @default.
- W2065384582 modified "2023-09-25" @default.
- W2065384582 title "Detector nonlinearity in frequency-domain fluorometry" @default.
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- W2065384582 doi "https://doi.org/10.1364/ao.32.000976" @default.
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