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• W2065386770 startingPage "911" @default.
• W2065386770 abstract "Tuberculosis, caused by Mycobacterium tuberculosis, continues to be one of the leading infectious diseases to humans. It is urgent to discover novel drug targets for the development of antitubercular agents. The 2-C-methyl-Derythritol-4-phosphate (MEP) pathway for isoprenoid biosynthesis has been considered as an attractive target for the discovery of novel antibiotics for its essentiality in bacteria and absence in mammals. MEP cytidyltransferase (IspD), the third-step enzyme of the pathway, catalyzes MEP and CTP to form 4-diphosphocytidyl-2-C-methylerythritol (CDP-ME) and PPi. In the work, ispD gene from M. tuberculosis H37Rv (MtIspD) was cloned and expressed. With N-terminal fusion of a histidine-tagged sequence, MtIspD could be purified to homogeneity by one-step nickel affinity chromatography. MtIspD exists as a homodimer with an apparent molecular mass of 52 kDa. Enzyme property analysis revealed that MtIspD has high specificity for pyrimidine bases and narrow divalent cation requirements, with maximal activity found in the presence of CTP and <TEX>$Mg^{2+}$</TEX>. The turnover number of MtIspD is <TEX>$3.4 s^{-1}$</TEX>. The Km for MEP and CTP are 43 and <TEX>$92{mu}M$</TEX>, respectively. Furthermore, MtIspD shows thermal instable above <TEX>$50^{circ}C$</TEX>. Circular dichroism spectra revealed that the alteration of tertiary conformation is closely related with sharp loss of enzyme activity at higher temperature. This study is expected to help better understand the features of IspD and provide useful information for the development of novel antibiotics to treat M. tuberculosis." @default.
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• W2065386770 date "2007-11-30" @default.
• W2065386770 modified "2023-09-24" @default.
• W2065386770 title "Biosynthesis of Isoprenoids: Characterization of a Functionally Active Recombinant 2-C-methyl-D-erythritol 4-phosphate Cytidyltransferase (IspD) from Mycobacterium tuberculosis H37Rv" @default.
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• W2065386770 doi "https://doi.org/10.5483/bmbrep.2007.40.6.911" @default.
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