FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2065432043> ?p ?o ?g. }

• W2065432043 endingPage "820" @default.
• W2065432043 startingPage "809" @default.
• W2065432043 abstract "Virtually nothing is known about the interaction of co-translationally active chaperones with nascent polypeptides and the resulting effects on peptide conformation and folding. We have explored this issue by NMR analysis of apomyoglobin N-terminal fragments of increasing length, taken as models for different stages of protein biosynthesis, in the absence and presence of the substrate binding domain of Escherichia coli Hsp70, DnaK-β. The incomplete polypeptides misfold and self-associate under refolding conditions. In the presence of DnaK-β, however, formation of the original self-associated species is completely or partially prevented. Chaperone interaction with incomplete protein chains promotes a globally unfolded dynamic DnaK-β-bound state, which becomes folding-competent only upon incorporation of the residues corresponding to the C-terminal H helix. The chaperone does not bind the full-length protein at equilibrium. However, its presence strongly disfavors the kinetic accessibility of misfolding side-routes available to the full-length chain. This work supports the role of DnaK as a “holder” for incomplete N-terminal polypeptides. However, as the chain approaches its full-length status, the tendency to intramolecularly bury non-polar surface efficiently outcompetes chaperone binding. Under these conditions, DnaK serves as a “folding enhancer” by supporting folding of a population of otherwise folding-incompetent full-length protein chains." @default.
• W2065432043 created "2016-06-24" @default.
• W2065432043 creator A5023224568 @default.
• W2065432043 creator A5036033946 @default.
• W2065432043 creator A5066903416 @default.
• W2065432043 date "2006-01-01" @default.
• W2065432043 modified "2023-10-18" @default.
• W2065432043 title "Effect of Hsp70 Chaperone on the Folding and Misfolding of Polypeptides Modeling an Elongating Protein Chain" @default.
• W2065432043 cites W1494494379 @default.
• W2065432043 cites W1498099361 @default.
• W2065432043 cites W1514163719 @default.
• W2065432043 cites W1541948779 @default.
• W2065432043 cites W1583407009 @default.
• W2065432043 cites W1973027077 @default.
• W2065432043 cites W1980967202 @default.
• W2065432043 cites W1981832868 @default.
• W2065432043 cites W1997630751 @default.
• W2065432043 cites W1997718936 @default.
• W2065432043 cites W2005356879 @default.
• W2065432043 cites W2015642465 @default.
• W2065432043 cites W2016088806 @default.
• W2065432043 cites W2020961473 @default.
• W2065432043 cites W2021006927 @default.
• W2065432043 cites W2030096182 @default.
• W2065432043 cites W2034012253 @default.
• W2065432043 cites W2037279684 @default.
• W2065432043 cites W2038345597 @default.
• W2065432043 cites W2039680648 @default.
• W2065432043 cites W2042180447 @default.
• W2065432043 cites W2051592058 @default.
• W2065432043 cites W2054634611 @default.
• W2065432043 cites W2056924571 @default.
• W2065432043 cites W2068227670 @default.
• W2065432043 cites W2074139343 @default.
• W2065432043 cites W2076945013 @default.
• W2065432043 cites W2082176639 @default.
• W2065432043 cites W2083281404 @default.
• W2065432043 cites W2083440806 @default.
• W2065432043 cites W2086348865 @default.
• W2065432043 cites W2089457735 @default.
• W2065432043 cites W2089618587 @default.
• W2065432043 cites W2090824354 @default.
• W2065432043 cites W2091608178 @default.
• W2065432043 cites W2093754939 @default.
• W2065432043 cites W2107031354 @default.
• W2065432043 cites W2109936231 @default.
• W2065432043 cites W2121340868 @default.
• W2065432043 cites W2129879061 @default.
• W2065432043 cites W2130479394 @default.
• W2065432043 cites W2138912150 @default.
• W2065432043 cites W2142094348 @default.
• W2065432043 cites W2169821755 @default.
• W2065432043 doi "https://doi.org/10.1016/j.jmb.2005.10.029" @default.
• W2065432043 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1570398" @default.
• W2065432043 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16309705" @default.
• W2065432043 hasPublicationYear "2006" @default.
• W2065432043 type Work @default.
• W2065432043 sameAs 2065432043 @default.
• W2065432043 citedByCount "28" @default.
• W2065432043 countsByYear W20654320432012 @default.
• W2065432043 countsByYear W20654320432013 @default.
• W2065432043 countsByYear W20654320432014 @default.
• W2065432043 countsByYear W20654320432016 @default.
• W2065432043 countsByYear W20654320432017 @default.
• W2065432043 countsByYear W20654320432018 @default.
• W2065432043 countsByYear W20654320432019 @default.
• W2065432043 countsByYear W20654320432020 @default.
• W2065432043 countsByYear W20654320432023 @default.
• W2065432043 crossrefType "journal-article" @default.
• W2065432043 hasAuthorship W2065432043A5023224568 @default.
• W2065432043 hasAuthorship W2065432043A5036033946 @default.
• W2065432043 hasAuthorship W2065432043A5066903416 @default.
• W2065432043 hasBestOaLocation W20654320432 @default.
• W2065432043 hasConcept C104317684 @default.
• W2065432043 hasConcept C119599485 @default.
• W2065432043 hasConcept C12554922 @default.
• W2065432043 hasConcept C127413603 @default.
• W2065432043 hasConcept C142724271 @default.
• W2065432043 hasConcept C162203774 @default.
• W2065432043 hasConcept C185592680 @default.
• W2065432043 hasConcept C204328495 @default.
• W2065432043 hasConcept C205260736 @default.
• W2065432043 hasConcept C2775962898 @default.
• W2065432043 hasConcept C2776545253 @default.
• W2065432043 hasConcept C547475151 @default.
• W2065432043 hasConcept C55493867 @default.
• W2065432043 hasConcept C68991219 @default.
• W2065432043 hasConcept C71924100 @default.
• W2065432043 hasConcept C8010536 @default.
• W2065432043 hasConcept C86803240 @default.
• W2065432043 hasConcept C87190427 @default.
• W2065432043 hasConcept C98924942 @default.
• W2065432043 hasConceptScore W2065432043C104317684 @default.
• W2065432043 hasConceptScore W2065432043C119599485 @default.
• W2065432043 hasConceptScore W2065432043C12554922 @default.
• W2065432043 hasConceptScore W2065432043C127413603 @default.
• W2065432043 hasConceptScore W2065432043C142724271 @default.
• W2065432043 hasConceptScore W2065432043C162203774 @default.

• next