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• W2065437086 abstract "A region of the Lymantria dispar multinucleocapsid nuclear polyhedrosis virus (LdMNPV) genome containing the homolog of the baculovirus ie -1 gene was identified using a series of overlapping cosmids and individual plasmids in a transient transcriptional expression assay. Sequence analysis of the active region identified two ORFs, one of which is 32% identical to AcMNPV ORF141 ( ie -0) and contains a putative splice donor site and the other of which is 29% identical to AcMNPV ie -1 and contains a highly conserved splice acceptor consensus sequence. Plasmids containing the LdMNPV ORF141 and ie -1 regions were able to stimulate expression of a GUS reporter gene, while plasmids containing the ie -1 region alone were inactive, suggesting that only the spliced, IE-0 form of the gene product is an active transactivator. Primer extension analysis confirmed the presence of spliced ie -0 mRNA transcripts starting at 6 hr and continuing throughout the time course of viral infection of the L. dispar cell line Ld652Y. Using a plasmid containing the ie -0 spliced form of the gene as a transactivator, hr 4, one of the eight homologous regions of LdMNPV, was shown to act as a transcriptional enhancer. In contrast, a reporter plasmid containing the AcMNPV hr 5 enhancer did not show increased activity when cotransfected with LdMNPV ie -0, suggesting that these enhancer sequences are viral specific. In a transient replication assay system, LdMNPV ie -0 acted as an essential replication gene, but LdMNPV ie -1 was inactive. These results indicate that splicing is required to obtain an active gene product in LdMNPV in the Ld652Y cell line." @default.
• W2065437086 created "2016-06-24" @default.
• W2065437086 creator A5049198481 @default.
• W2065437086 creator A5087751268 @default.
• W2065437086 date "1997-08-01" @default.
• W2065437086 modified "2023-09-27" @default.
• W2065437086 title "Splicing Is Required for Transactivation by the Immediate Early Gene 1 of theLymantria disparMultinucleocapsid Nuclear Polyhedrosis Virus" @default.
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• W2065437086 doi "https://doi.org/10.1006/viro.1997.8687" @default.
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