Matches in SemOpenAlex for { <https://semopenalex.org/work/W2065441385> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W2065441385 abstract "Grapevine fanleaf nepovirus (GFLV) has a bipartite plus-sense RNA genome. Its structural and functional proteins originate from polyprotein maturation by at least one virus-encoded proteinase. Here we describe the cloning of the 24-kDa proteinase cistron located between the virus-linked protein (VPg) and the RNA-dependent RNA polymerase cistron in GFLV RNA1 (nucleotides 3966 to 4622). Proteinase expressed from this clone is able to cleave GFLV polyprotein P2 in order to produce the coat protein and a 66-kDa protein which is further processed to the 38-kDa presumed movement protein. The GFLV 24-kDa proteinase sequence contains sequence similarities with other nepovirus and comovirus proteinases, particularly at the level of the conserved domains corresponding to the hypothetical catalytic triad and to the substrate-binding pocket (amino acids 192 to 200). Site-directed mutagenesis of residues His43, Glu87, and Leu197 abolished proteinase activity. Inactivation of the enzyme is also observed if the catalytic residue Cys179 was substituted by isoleucine, but replacement by a serine at the same position produced a mutant with an activity identical to that of native proteinase. All our data show that GFLV cysteine proteinase presents structure similarities to the proteinases of cowpea mosaic virus and potyviruses but is most closely related to trypsin." @default.
- W2065441385 created "2016-06-24" @default.
- W2065441385 creator A5002532606 @default.
- W2065441385 creator A5075959068 @default.
- W2065441385 date "1992-10-01" @default.
- W2065441385 modified "2023-09-30" @default.
- W2065441385 title "Effects of site-directed mutagenesis on the presumed catalytic triad and substrate-binding pocket of grapevine fanleaf nepovirus 24-kDa proteinase" @default.
- W2065441385 cites W1487649776 @default.
- W2065441385 cites W1528509576 @default.
- W2065441385 cites W1964612834 @default.
- W2065441385 cites W1964663543 @default.
- W2065441385 cites W1969324434 @default.
- W2065441385 cites W1987961630 @default.
- W2065441385 cites W1988255003 @default.
- W2065441385 cites W2007058656 @default.
- W2065441385 cites W2008269336 @default.
- W2065441385 cites W2008346804 @default.
- W2065441385 cites W2009310436 @default.
- W2065441385 cites W2015165754 @default.
- W2065441385 cites W2030461289 @default.
- W2065441385 cites W2031745921 @default.
- W2065441385 cites W2051517579 @default.
- W2065441385 cites W2053702224 @default.
- W2065441385 cites W2062028851 @default.
- W2065441385 cites W2070027338 @default.
- W2065441385 cites W2087620078 @default.
- W2065441385 cites W2096716204 @default.
- W2065441385 cites W2099937013 @default.
- W2065441385 cites W2112636022 @default.
- W2065441385 cites W2126475232 @default.
- W2065441385 cites W2141264624 @default.
- W2065441385 doi "https://doi.org/10.1016/0042-6822(92)90931-e" @default.
- W2065441385 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1519363" @default.
- W2065441385 hasPublicationYear "1992" @default.
- W2065441385 type Work @default.
- W2065441385 sameAs 2065441385 @default.
- W2065441385 citedByCount "41" @default.
- W2065441385 countsByYear W20654413852013 @default.
- W2065441385 countsByYear W20654413852017 @default.
- W2065441385 countsByYear W20654413852018 @default.
- W2065441385 countsByYear W20654413852021 @default.
- W2065441385 countsByYear W20654413852023 @default.
- W2065441385 crossrefType "journal-article" @default.
- W2065441385 hasAuthorship W2065441385A5002532606 @default.
- W2065441385 hasAuthorship W2065441385A5075959068 @default.
- W2065441385 hasConcept C104317684 @default.
- W2065441385 hasConcept C153911025 @default.
- W2065441385 hasConcept C159047783 @default.
- W2065441385 hasConcept C199083928 @default.
- W2065441385 hasConcept C2776994594 @default.
- W2065441385 hasConcept C41258723 @default.
- W2065441385 hasConcept C55493867 @default.
- W2065441385 hasConcept C67705224 @default.
- W2065441385 hasConcept C86803240 @default.
- W2065441385 hasConceptScore W2065441385C104317684 @default.
- W2065441385 hasConceptScore W2065441385C153911025 @default.
- W2065441385 hasConceptScore W2065441385C159047783 @default.
- W2065441385 hasConceptScore W2065441385C199083928 @default.
- W2065441385 hasConceptScore W2065441385C2776994594 @default.
- W2065441385 hasConceptScore W2065441385C41258723 @default.
- W2065441385 hasConceptScore W2065441385C55493867 @default.
- W2065441385 hasConceptScore W2065441385C67705224 @default.
- W2065441385 hasConceptScore W2065441385C86803240 @default.
- W2065441385 hasLocation W20654413851 @default.
- W2065441385 hasLocation W20654413852 @default.
- W2065441385 hasOpenAccess W2065441385 @default.
- W2065441385 hasPrimaryLocation W20654413851 @default.
- W2065441385 hasRelatedWork W1974103390 @default.
- W2065441385 hasRelatedWork W1986237405 @default.
- W2065441385 hasRelatedWork W2089822958 @default.
- W2065441385 hasRelatedWork W2089959240 @default.
- W2065441385 hasRelatedWork W2137677829 @default.
- W2065441385 hasRelatedWork W2138538655 @default.
- W2065441385 hasRelatedWork W2406802924 @default.
- W2065441385 hasRelatedWork W2411232233 @default.
- W2065441385 hasRelatedWork W2418539106 @default.
- W2065441385 hasRelatedWork W2502728792 @default.
- W2065441385 isParatext "false" @default.
- W2065441385 isRetracted "false" @default.
- W2065441385 magId "2065441385" @default.
- W2065441385 workType "article" @default.