Matches in SemOpenAlex for { <https://semopenalex.org/work/W2065448935> ?p ?o ?g. }
Showing items 1 to 70 of
70
with 100 items per page.
- W2065448935 endingPage "279" @default.
- W2065448935 startingPage "273" @default.
- W2065448935 abstract "A gene encoding a nicotinamide mononucleotide (NMN) adenylyltransferase (NMNAT, EC 2.7.7.1) homologue was identified via genome sequencing in the anaerobic hyperthermophilic archaeon Pyrococcus horikoshii OT-3. The gene encoded a protein of 186 amino acids with a molecular weight of 21,391. The deduced amino acid sequence of the gene showed 59% identities to the NMNAT from Methanococcus jannaschii. The gene was overexpressed in Escherichia coli, and the produced enzyme was purified to homogeneity. Characterization of the enzyme revealed that it is an extremely thermostable NMNAT; the activity was not lost after incubation at 80 °C for 30 min. The native molecular mass was estimated to be 77 kDa. The Km values for ATP and NMN were calculated to be 0.056 and 0.061 mM, respectively. The optimum temperature of the reaction was estimated to be around 90 °C. The adenylyl group donor specificity was examined by high-performance liquid chromatography (HPLC). At 70 °C, ATP was a prominent donor. However, above 80 °C, a relatively small, but significant, NMNAT activity was detected when ATP was replaced by ADP or AMP in the reaction mixture. To date, an NMNAT that utilizes ADP or AMP as an adenylyl group donor has not been found. The present study provides interesting information in which a di- or mono-phosphate nucleotide can be utilized by adenylyltransferase at high temperature." @default.
- W2065448935 created "2016-06-24" @default.
- W2065448935 creator A5002278218 @default.
- W2065448935 creator A5012026574 @default.
- W2065448935 creator A5070568461 @default.
- W2065448935 creator A5087399898 @default.
- W2065448935 creator A5087777648 @default.
- W2065448935 date "2003-09-01" @default.
- W2065448935 modified "2023-10-17" @default.
- W2065448935 title "A nicotinamide mononucleotide adenylyltransferase with unique adenylyl group donor specificity from a hyperthermophilic archaeon, Pyrococcus horikoshii OT-3" @default.
- W2065448935 cites W1486865933 @default.
- W2065448935 cites W1497505440 @default.
- W2065448935 cites W1532289340 @default.
- W2065448935 cites W2051523955 @default.
- W2065448935 cites W2055043387 @default.
- W2065448935 cites W2068807529 @default.
- W2065448935 cites W2100837269 @default.
- W2065448935 cites W2128635872 @default.
- W2065448935 cites W2150782144 @default.
- W2065448935 cites W2156099263 @default.
- W2065448935 cites W2969273703 @default.
- W2065448935 doi "https://doi.org/10.1016/s1381-1177(03)00090-0" @default.
- W2065448935 hasPublicationYear "2003" @default.
- W2065448935 type Work @default.
- W2065448935 sameAs 2065448935 @default.
- W2065448935 citedByCount "4" @default.
- W2065448935 countsByYear W20654489352018 @default.
- W2065448935 crossrefType "journal-article" @default.
- W2065448935 hasAuthorship W2065448935A5002278218 @default.
- W2065448935 hasAuthorship W2065448935A5012026574 @default.
- W2065448935 hasAuthorship W2065448935A5070568461 @default.
- W2065448935 hasAuthorship W2065448935A5087399898 @default.
- W2065448935 hasAuthorship W2065448935A5087777648 @default.
- W2065448935 hasConcept C181199279 @default.
- W2065448935 hasConcept C185592680 @default.
- W2065448935 hasConcept C2776901170 @default.
- W2065448935 hasConcept C2777088662 @default.
- W2065448935 hasConcept C2778332380 @default.
- W2065448935 hasConcept C55493867 @default.
- W2065448935 hasConcept C75520062 @default.
- W2065448935 hasConcept C86803240 @default.
- W2065448935 hasConceptScore W2065448935C181199279 @default.
- W2065448935 hasConceptScore W2065448935C185592680 @default.
- W2065448935 hasConceptScore W2065448935C2776901170 @default.
- W2065448935 hasConceptScore W2065448935C2777088662 @default.
- W2065448935 hasConceptScore W2065448935C2778332380 @default.
- W2065448935 hasConceptScore W2065448935C55493867 @default.
- W2065448935 hasConceptScore W2065448935C75520062 @default.
- W2065448935 hasConceptScore W2065448935C86803240 @default.
- W2065448935 hasIssue "2-6" @default.
- W2065448935 hasLocation W20654489351 @default.
- W2065448935 hasOpenAccess W2065448935 @default.
- W2065448935 hasPrimaryLocation W20654489351 @default.
- W2065448935 hasRelatedWork W1218434330 @default.
- W2065448935 hasRelatedWork W1546333328 @default.
- W2065448935 hasRelatedWork W2003046999 @default.
- W2065448935 hasRelatedWork W2077374667 @default.
- W2065448935 hasRelatedWork W2121874177 @default.
- W2065448935 hasRelatedWork W2145833950 @default.
- W2065448935 hasRelatedWork W2158094397 @default.
- W2065448935 hasRelatedWork W2531686927 @default.
- W2065448935 hasRelatedWork W3120238875 @default.
- W2065448935 hasRelatedWork W4239637547 @default.
- W2065448935 hasVolume "23" @default.
- W2065448935 isParatext "false" @default.
- W2065448935 isRetracted "false" @default.
- W2065448935 magId "2065448935" @default.
- W2065448935 workType "article" @default.