FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2065452285> ?p ?o ?g. }

• W2065452285 endingPage "158" @default.
• W2065452285 startingPage "144" @default.
• W2065452285 abstract "We tested and clinically validated a targeted next-generation sequencing (NGS) mutation panel using 80 formalin-fixed, paraffin-embedded (FFPE) tumor samples. Forty non-small cell lung carcinoma (NSCLC), 30 melanoma, and 30 gastrointestinal (12 colonic, 10 gastric, and 8 pancreatic adenocarcinoma) FFPE samples were selected from laboratory archives. After appropriate specimen and nucleic acid quality control, 80 NGS libraries were prepared using the Illumina TruSight tumor (TST) kit and sequenced on the Illumina MiSeq. Sequence alignment, variant calling, and sequencing quality control were performed using vendor software and laboratory-developed analysis workflows. TST generated ≥500× coverage for 98.4% of the 13,952 targeted bases. Reproducible and accurate variant calling was achieved at ≥5% variant allele frequency with 8 to 12 multiplexed samples per MiSeq flow cell. TST detected 112 variants overall, and confirmed all known single-nucleotide variants (n = 27), deletions (n = 5), insertions (n = 3), and multinucleotide variants (n = 3). TST detected at least one variant in 85.0% (68/80), and two or more variants in 36.2% (29/80), of samples. TP53 was the most frequently mutated gene in NSCLC (13 variants; 13/32 samples), gastrointestinal malignancies (15 variants; 13/25 samples), and overall (30 variants; 28/80 samples). BRAF mutations were most common in melanoma (nine variants; 9/23 samples). Clinically relevant NGS data can be obtained from routine clinical FFPE solid tumor specimens using TST, benchtop instruments, and vendor-supplied bioinformatics pipelines." @default.
• W2065452285 created "2016-06-24" @default.
• W2065452285 creator A5023581311 @default.
• W2065452285 creator A5040717966 @default.
• W2065452285 creator A5046410639 @default.
• W2065452285 creator A5066607924 @default.
• W2065452285 date "2014-09-01" @default.
• W2065452285 modified "2023-10-14" @default.
• W2065452285 title "Section I: Integrating laboratory medicine with tissue specimens" @default.
• W2065452285 cites W126643673 @default.
• W2065452285 cites W153747871 @default.
• W2065452285 cites W1580924836 @default.
• W2065452285 cites W159558800 @default.
• W2065452285 cites W1694119819 @default.
• W2065452285 cites W1773589956 @default.
• W2065452285 cites W1876623834 @default.
• W2065452285 cites W1908711430 @default.
• W2065452285 cites W1970438376 @default.
• W2065452285 cites W1974685860 @default.
• W2065452285 cites W1975075489 @default.
• W2065452285 cites W1977950576 @default.
• W2065452285 cites W1980661152 @default.
• W2065452285 cites W1984792432 @default.
• W2065452285 cites W1986067358 @default.
• W2065452285 cites W1998321506 @default.
• W2065452285 cites W2000559212 @default.
• W2065452285 cites W2003712825 @default.
• W2065452285 cites W2005160335 @default.
• W2065452285 cites W2015823489 @default.
• W2065452285 cites W2022495797 @default.
• W2065452285 cites W2022785557 @default.
• W2065452285 cites W2023378387 @default.
• W2065452285 cites W2029368081 @default.
• W2065452285 cites W2044752896 @default.
• W2065452285 cites W2046288207 @default.
• W2065452285 cites W2046607138 @default.
• W2065452285 cites W2048553228 @default.
• W2065452285 cites W2049656505 @default.
• W2065452285 cites W2050814329 @default.
• W2065452285 cites W2051380427 @default.
• W2065452285 cites W2054636737 @default.
• W2065452285 cites W2084985708 @default.
• W2065452285 cites W2085448923 @default.
• W2065452285 cites W2088545396 @default.
• W2065452285 cites W2096460732 @default.
• W2065452285 cites W2096504879 @default.
• W2065452285 cites W2108480019 @default.
• W2065452285 cites W2111404068 @default.
• W2065452285 cites W2118526609 @default.
• W2065452285 cites W2124558732 @default.
• W2065452285 cites W2127250774 @default.
• W2065452285 cites W2128542677 @default.
• W2065452285 cites W2130153722 @default.
• W2065452285 cites W2134447998 @default.
• W2065452285 cites W2136482257 @default.
• W2065452285 cites W2137983323 @default.
• W2065452285 cites W2138709799 @default.
• W2065452285 cites W2140901845 @default.
• W2065452285 cites W2141450265 @default.
• W2065452285 cites W2148565184 @default.
• W2065452285 cites W2154813452 @default.
• W2065452285 cites W2157694607 @default.
• W2065452285 cites W2160860814 @default.
• W2065452285 cites W2162786929 @default.
• W2065452285 cites W2168917479 @default.
• W2065452285 cites W2514360855 @default.
• W2065452285 cites W295191605 @default.
• W2065452285 cites W47570996 @default.
• W2065452285 doi "https://doi.org/10.1016/j.currproblcancer.2014.08.004" @default.
• W2065452285 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25239562" @default.
• W2065452285 hasPublicationYear "2014" @default.
• W2065452285 type Work @default.
• W2065452285 sameAs 2065452285 @default.
• W2065452285 citedByCount "4" @default.
• W2065452285 countsByYear W20654522852015 @default.
• W2065452285 countsByYear W20654522852016 @default.
• W2065452285 countsByYear W20654522852017 @default.
• W2065452285 crossrefType "journal-article" @default.
• W2065452285 hasAuthorship W2065452285A5023581311 @default.
• W2065452285 hasAuthorship W2065452285A5040717966 @default.
• W2065452285 hasAuthorship W2065452285A5046410639 @default.
• W2065452285 hasAuthorship W2065452285A5066607924 @default.
• W2065452285 hasConcept C104317684 @default.
• W2065452285 hasConcept C121608353 @default.
• W2065452285 hasConcept C126322002 @default.
• W2065452285 hasConcept C142724271 @default.
• W2065452285 hasConcept C2776256026 @default.
• W2065452285 hasConcept C2781182431 @default.
• W2065452285 hasConcept C51679486 @default.
• W2065452285 hasConcept C54355233 @default.
• W2065452285 hasConcept C71924100 @default.
• W2065452285 hasConcept C86803240 @default.
• W2065452285 hasConceptScore W2065452285C104317684 @default.
• W2065452285 hasConceptScore W2065452285C121608353 @default.
• W2065452285 hasConceptScore W2065452285C126322002 @default.
• W2065452285 hasConceptScore W2065452285C142724271 @default.
• W2065452285 hasConceptScore W2065452285C2776256026 @default.
• W2065452285 hasConceptScore W2065452285C2781182431 @default.

• next