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- W2065506618 abstract "Complementary DNA regions coding for two different mature goldfish growth hormones (gfGH-I and gfGH-II) with four and five cysteine residues were cloned into the bacterial expression vector, pRSETA. The recombinant gfGH-I (five cysteines) and -II (four cysteines) were produced in Escherichia coli as the fusion proteins carrying N-terminal 6XHis tag, which facilitates purification by using metal chelating affinity chromatography under denaturing condition with urea. The recombinant hormones were further refolded by gradually removing the urea. Native gfGH was also purified from goldfish pituitary glands and served as a positive control in the present study. The native and recombinant hormones were tested in goldfish hepatic radioreceptor assay and in vitro Spi 2.1 promoter activation assay. Our results showed that the two recombinant gfGHs are biologically active, and they have similar biological activities despite their having different cysteine contents." @default.
- W2065506618 created "2016-06-24" @default.
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- W2065506618 date "2003-08-01" @default.
- W2065506618 modified "2023-09-25" @default.
- W2065506618 title "Recombinant goldfish growth hormones (gfGH-I and -II) expressed in Escherichia coli have similar biological activities" @default.
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- W2065506618 doi "https://doi.org/10.1016/s1095-6433(03)00143-0" @default.
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