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• W2065536296 startingPage "1237" @default.
• W2065536296 abstract "In this study we examined the potential of a novel thermoreversible gelation polymer (TGP) to act as a 3-D hydrogel scaffold and deliver both chondrocytes and growth factors. Chondrocytes obtained from bovine articular cartilage were studied as a suspension in TGP chilled to 4°C, in the presence or absence of the growth factors IGF-1 and/or TGF β2. The cold cell/aqueous suspensions were injected into a cylindrical mold and cultured at 37°C for up to 16 weeks. Specimens obtained at 12 and 16 weeks were semitranslucent and elastic. The matrices surrounding the chondrocytes were histologically positive to Safranin-O staining and type II collagen staining. The glycosaminoglycan and hydroxyproline contents in the specimens increased as a function of time and because of the presence of growth factors; those cultured with growth factors produced significantly more of these substances than those cultured without. We have concluded that TGP has potential as a scaffold material in the generation of tissue-engineered cartilage in vitro." @default.
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• W2065536296 date "2006-05-01" @default.
• W2065536296 modified "2023-09-30" @default.
• W2065536296 title "<i>In Vitro</i> Culture of Chondrocytes in a Novel Thermoreversible Gelation Polymer Scaffold Containing Growth Factors" @default.
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• W2065536296 doi "https://doi.org/10.1089/ten.2006.12.1237" @default.
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