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- W2065544765 abstract "A simple method for the purification of sheep spleen deoxyribonuclease II (deoxyribonucleate 3′-nucleotidohydrolase, EC 3.1.4.6) is described. Spleen is dispersed in water, the homogenate acidified, clarified, heated at 50 ° for 1 hr at pH 3, and subjected to chromatography on CM-cellulose, Sephadex, and DEAE-cellulose. The specific activity of the final product is approximately 4000 times greater than that of the homogenate. The deoxyribonuclease (DNase) II is free from ribonucleases, phosphatases, spleen exonuclease, and nonspecific phosphodiesterase. It appears homogeneous after gel chromatography, density-gradient centrifugation in sucrose, and acrylamide gel electrophoresis." @default.
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- W2065544765 modified "2023-09-26" @default.
- W2065544765 title "Purification of deoxyribonuclease II from sheep spleen" @default.
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- W2065544765 doi "https://doi.org/10.1016/0003-9861(70)90058-5" @default.
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