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• W2065554849 abstract "Gd-diethylenetriamine pentaacetic acid-bismethylamide, Gd(DTPA-BMA), is shown to be a reagent suitable for the identification of protein surfaces. Compared to the conventionally used spin-label TEMPOL, Gd(DTPA-BMA) is a stronger relaxation agent, requiring lesser concentrations to achieve the same paramagnetic relaxation enhancement of solvent-exposed protein protons. It is also less hydrophobic and therefore less prone to specific binding to proteins. Relaxation enhancements predicted by a second-sphere interaction model correlated with experimental data recorded with ubiquitin, while the correlation with corresponding data recorded with TEMPOL was poor." @default.
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• W2065554849 date "2001-12-22" @default.
• W2065554849 modified "2023-09-30" @default.
• W2065554849 title "Identification of Protein Surfaces by NMR Measurements with a Paramagnetic Gd(III) Chelate" @default.
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• W2065554849 doi "https://doi.org/10.1021/ja016985h" @default.
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