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• W2065562185 abstract "Two enterotoxigenic Escherichia coli (ETEC) strains (H10407 and 4011-1) were charectrized by random amplification of polymorphic DNA (RAPD) profiles using 10-mer oligonucleotides with diverse GC content. All tested primers yielded arrays of amplified DNA products ranging in size from 200 to 3000 bp. The effects of annealing temperature, template concentration and GC content of the primers were evaluated and an optimal reaction procedure was established. Application of the RAPD analysis to ten ETEC strains belonging to five different serotypes showed that strains of the same serotype shared identical or almost identical band profiles, suggesting a similar genetic composition. The use of RAPD profiles as a tool in epidemiological analysis of ETEC is discussed. Deux souches (H10407 et 4011-1) entérotoxigènes de Escherichia coli (ETEC) ont été caractérisées par AP-PCR ou RAPD au moyen d'oligonucléotides 10-mériques ayant diverses teneurs en GC. Toutes les amorces utilisées avaient des gammes d'ADN amplifié de 200 à 3000 bp. Les effets des diverses variables techniques et des GC % ont été évalués et un protocole optimal a été établi. L'application de la méthode RAPD à l'analyse des 10 souches ETEC appartenant à 5 sérotypes montre que les souches de sérotype identique ont des profils identiques ou presque identiques, suggérant une similarité génomique. L'utilisation des profils RAPD comme outil d'analyse épidémiologique des ETECs est discutée." @default.
• W2065562185 created "2016-06-24" @default.
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• W2065562185 date "1996-01-01" @default.
• W2065562185 modified "2023-10-18" @default.
• W2065562185 title "Characterization of enterotoxigenic Escherichia coli by random amplification of polymorphic DNA" @default.
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• W2065562185 doi "https://doi.org/10.1016/0923-2508(96)80217-8" @default.
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