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- W2065575945 abstract "Using Baginski and Zak's (12) method for determining inorganic orthophosphate as a starting point, a number of conditions which influence the accuracy and precision of the determination of pyrophosphatase activity have been shown: nonenzyme-catalyzed acid hydrolysis by protein precipitation agents and molybdate-catalyzed hydrolysis of pyrophosphate together with interference in the determination of orthophosphate by the substrate pyrophosphate and other components from the reaction mixture, Tris, chloride, acetate, citrate, boric acid. With regard to these sources of error, a method is described for determining pyrophosphatase activity, and its reliability is investigated." @default.
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- W2065575945 date "1972-09-01" @default.
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- W2065575945 title "Colorimetric determination of orthophosphate in the assay of inorganic pyrophosphatase activity" @default.
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- W2065575945 doi "https://doi.org/10.1016/0003-2697(72)90240-0" @default.
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