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- W2065584059 abstract "Accumulation and effects of cadmium were investigated in Chironomus thummi larvae exposed to 10, 100 and 250 μg radiolabeled Cd/1 for up to 4 days. (1) After 4 days, average cadmium accumulation was 6.6 ng Cd/mg dry weight (10 μg Cd/1 exposure) and 177 ng Cd/mg dry weight (250 μg Cd/1 exposure). (2) Dissection studies showed that by 32 hr of exposure to both cadmium concentrations, 63.5–81.4% of accumulated cadmium was confined to the posterior midgut epithelium. Light microscope autoradiography similarly showed accumulations of cadmium in posterior midgut epithelium and smaller amounts in fat body and muscle. Little cadmium was associated with Malphigian tubules, haemocoel, anterior midgut or exoskeleton. (3) After exposure to 10 or 250 μg Cd/1, 60–75% of cadmium in ultracentrifuged homogenates of whole animals or dissected guts was associated with the resulting supernatant. When supernatants were further analyzed by gel chromatography, cadmium eluted with both a high and low molecular weight peak. The relative proportions of cadmium in the two peaks varied with concentration and length of exposure. (4) Transmission electron microscopy of posterior midgut cells from animals exposed to cadmium demonstrated frequent mitochondrial lesions. Exposure to high cadmium concentrations caused some posterior midgut cells to undergo generalized structural degeneration." @default.
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- W2065584059 date "1986-01-01" @default.
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- W2065584059 title "Accumulation of cadmium by the fourth instar larva of the fly Chironomus thummi" @default.
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- W2065584059 doi "https://doi.org/10.1016/0040-8166(86)90059-5" @default.
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