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- W2065587139 abstract "In previous experiments we have established that various strains of Bordetella pertussis are lysogenic and release phage under the influence of mitomycin C. Phage release was accomplished by incubating B. pertussis bacteria with mitomycin C (4.5 ng/ml) for 1 h at 36°C, followed by washing, resuspension in Cohen-Wheeler medium [1], further incubation for 18 h at 36°C on a shaker, and addition of chloroform. The phage could be demonstrated by titration [2] on a strain of B. parapertussis, and further propagated on the same strain for electron microscopic observation [3]. Since temperate phage can also be released spontaneously, we wanted to test for phage release from several strains of B. pertussis, which are commonly used for vaccine production." @default.
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- W2065587139 date "1981-11-01" @default.
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- W2065587139 title "Spontaneous release of phage from strains ofBordetella pertussisused for vaccine production" @default.
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- W2065587139 doi "https://doi.org/10.1111/j.1574-6968.1981.tb07647.x" @default.
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