Matches in SemOpenAlex for { <https://semopenalex.org/work/W2065588602> ?p ?o ?g. }
Showing items 1 to 89 of
89
with 100 items per page.
- W2065588602 endingPage "1357" @default.
- W2065588602 startingPage "1353" @default.
- W2065588602 abstract "Long-term lamivudine treatment for chronic hepatitis B virus (HBV) infection induces the emergence of lamivudine resistant HBV YMDD mutant strains. The aim of the present study was to observe the clone evolution of YMDD wild type and mutant strains in pretreatment and post-treatment samples during lamivudine therapy and analyze their clinical significance.Ten serum samples (five before and five after 48 weeks of therapy) obtained from five patients chronically infected with HBV and treated with lamivudine were studied. Part of the HBV polymerase gene flanking the YMDD motif was sequenced after polymerase chain reaction (PCR) amplification. Meanwhile, 20-24 clones were selected at random from each sample and YMDD wild type and mutant strains were detected by real-time fluorimetry PCR established in our laboratory.The YMDD mutants were not detectable in all five patients before treatment and were found in four patients after 48 weeks of therapy by sequencing directly on PCR products. Analysis of individual clones showed that the ratios of mutant strains in each of the five patients were 0, 9.5, 0, 4.5 and 5.6%, respectively, before therapy and 100, 100, 65, 100 and 0%, respectively, after 48 weeks of therapy. M552I was detected before therapy in one patient but M552V became the domain strain after therapy. Until 52 weeks of therapy, serum HBV DNA and alanine aminotransferase (ALT) breakthrough were found in two of the four patients with YMDD mutations. The fifth patient experienced breakthrough of ALT but HBV DNA remained undetectable.The mutant strains of YMDD motif of HBV polymerase could be found in patients before lamivudine treatment, indicating that antiviral therapy allows the rapid selection of resistant strains. The replication ability of the M552V mutant strain might be stronger than that of the M552I mutant strain." @default.
- W2065588602 created "2016-06-24" @default.
- W2065588602 creator A5017774638 @default.
- W2065588602 creator A5019604775 @default.
- W2065588602 creator A5035441171 @default.
- W2065588602 creator A5058298924 @default.
- W2065588602 creator A5061060498 @default.
- W2065588602 creator A5068581809 @default.
- W2065588602 creator A5071389917 @default.
- W2065588602 date "2003-11-20" @default.
- W2065588602 modified "2023-10-14" @default.
- W2065588602 title "Evolution of wild type and mutants of the YMDD motif of hepatitis B virus polymerase during lamivudine therapy" @default.
- W2065588602 cites W1857344328 @default.
- W2065588602 cites W1985577568 @default.
- W2065588602 cites W1991592276 @default.
- W2065588602 cites W1998549562 @default.
- W2065588602 cites W1999864391 @default.
- W2065588602 cites W2045953763 @default.
- W2065588602 cites W2051090621 @default.
- W2065588602 cites W2052700689 @default.
- W2065588602 cites W2068399780 @default.
- W2065588602 cites W2122863469 @default.
- W2065588602 cites W2136425954 @default.
- W2065588602 cites W2161472068 @default.
- W2065588602 cites W2166826446 @default.
- W2065588602 cites W2169661908 @default.
- W2065588602 cites W2338794667 @default.
- W2065588602 cites W3152231134 @default.
- W2065588602 cites W4362208329 @default.
- W2065588602 doi "https://doi.org/10.1046/j.1440-1746.2003.03176.x" @default.
- W2065588602 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14675262" @default.
- W2065588602 hasPublicationYear "2003" @default.
- W2065588602 type Work @default.
- W2065588602 sameAs 2065588602 @default.
- W2065588602 citedByCount "15" @default.
- W2065588602 countsByYear W20655886022012 @default.
- W2065588602 countsByYear W20655886022013 @default.
- W2065588602 countsByYear W20655886022016 @default.
- W2065588602 countsByYear W20655886022022 @default.
- W2065588602 crossrefType "journal-article" @default.
- W2065588602 hasAuthorship W2065588602A5017774638 @default.
- W2065588602 hasAuthorship W2065588602A5019604775 @default.
- W2065588602 hasAuthorship W2065588602A5035441171 @default.
- W2065588602 hasAuthorship W2065588602A5058298924 @default.
- W2065588602 hasAuthorship W2065588602A5061060498 @default.
- W2065588602 hasAuthorship W2065588602A5068581809 @default.
- W2065588602 hasAuthorship W2065588602A5071389917 @default.
- W2065588602 hasConcept C104317684 @default.
- W2065588602 hasConcept C143065580 @default.
- W2065588602 hasConcept C159047783 @default.
- W2065588602 hasConcept C2522874641 @default.
- W2065588602 hasConcept C2777869810 @default.
- W2065588602 hasConcept C2780593183 @default.
- W2065588602 hasConcept C49105822 @default.
- W2065588602 hasConcept C54355233 @default.
- W2065588602 hasConcept C71924100 @default.
- W2065588602 hasConcept C86803240 @default.
- W2065588602 hasConceptScore W2065588602C104317684 @default.
- W2065588602 hasConceptScore W2065588602C143065580 @default.
- W2065588602 hasConceptScore W2065588602C159047783 @default.
- W2065588602 hasConceptScore W2065588602C2522874641 @default.
- W2065588602 hasConceptScore W2065588602C2777869810 @default.
- W2065588602 hasConceptScore W2065588602C2780593183 @default.
- W2065588602 hasConceptScore W2065588602C49105822 @default.
- W2065588602 hasConceptScore W2065588602C54355233 @default.
- W2065588602 hasConceptScore W2065588602C71924100 @default.
- W2065588602 hasConceptScore W2065588602C86803240 @default.
- W2065588602 hasIssue "12" @default.
- W2065588602 hasLocation W20655886021 @default.
- W2065588602 hasLocation W20655886022 @default.
- W2065588602 hasOpenAccess W2065588602 @default.
- W2065588602 hasPrimaryLocation W20655886021 @default.
- W2065588602 hasRelatedWork W1964792389 @default.
- W2065588602 hasRelatedWork W1993993021 @default.
- W2065588602 hasRelatedWork W2028101824 @default.
- W2065588602 hasRelatedWork W2052260190 @default.
- W2065588602 hasRelatedWork W2094115803 @default.
- W2065588602 hasRelatedWork W2307814414 @default.
- W2065588602 hasRelatedWork W2349537382 @default.
- W2065588602 hasRelatedWork W2357335082 @default.
- W2065588602 hasRelatedWork W2361330668 @default.
- W2065588602 hasRelatedWork W2380203252 @default.
- W2065588602 hasVolume "18" @default.
- W2065588602 isParatext "false" @default.
- W2065588602 isRetracted "false" @default.
- W2065588602 magId "2065588602" @default.
- W2065588602 workType "article" @default.