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W2065591738 abstract "Proceedings: AACR 101st Annual Meeting 2010‐‐ Apr 17‐21, 2010; Washington, DCMicroRNAs, ∼22 nt non-coding small RNAs, have been newly focused as a potential cancer diagnostic biomarker and therapy target, because aberrant expressions of miRNAs have been often found to involve in tumorigenesis, cancer development and progression. For instance, microRNA-101 (miR-101) is significantly down-regulated in several types of cancer. In this study, we investigated the function of miR-101 on regulation of gene expression in cancer cells. A stable miR-101 over-expressed prostate cancer cell line (BPH1CAFTDmiR101) was established by using lentivirus-delivery system. Over-expressed miR-101 level in the stable transfected cell can be detected after the 5th or 100th cell culture passages and approximately 10-fold higher than non-transfected BPH1CAFTD cells. We found that over-expressed miR-101 suppressed cyclooxygenase-2 (COX-2) protein expression in BPH1CAFTDmiR101 cells. The miR-101 targeted gene was determined by using luciferase reporter gene, which carry 3’-UTR ([NM_000963][1] 3’-UTR 1735-1741) of COX-2 mRNA. The results indicated that miR-101 directly targets COX-2 gene and down-regulates COX-2 protein expression by binding to 3’-UTR of COX-2 mRNA. Interestedly, the reduced COX-2 protein expression in BPH1CAFTDmiR101can be restored by transient transfection of COX-2 3′UTR fragment. Compared with BPH1CAFTD cells, BPH1CAFTDmiR101 proliferation and colony formation were significantly inhibited, and the expressions of a series of cell proliferation-related protein were significantly changed, such as the levels of EGFR, PCNA, and Bcl-2 were decreased and the level of p53 was increased. In addition, MMP-15 protein level, a metastasis related protein, was also noticeably reduced. Taking together, our finings demonstrate that miR-101 inhibits prostate cancer cell proliferation by directly targeting COX-2 to down-regulating its protein level. The miR-101 may function as tumor suppressor and a potential target for cancer diagnosis and therapy.Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 2102. [1]: /lookup/external-ref?link_type=GEN&access_num=NM_000963&atom=%2Fcanres%2F70%2F8_Supplement%2F2102.atom" @default.
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W2065591738 date "2010-04-15" @default.
W2065591738 modified "2023-09-25" @default.
W2065591738 title "Abstract 2102: Exogenous microRNA-101 suppresses cell proliferation by downregulation of cyclooxygenase-2 expression in prostate cancer cell line" @default.
W2065591738 doi "https://doi.org/10.1158/1538-7445.am10-2102" @default.
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