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• W2065602923 abstract "Targeted gene knockout using engineered nucleases such as transcription activator like-effector nucleases (TALENs) is a gold standard for investigating the functions of a gene of interest. Although most TALENs can cleave chromosomal DNA efficiently, the activities of designed TALENs are not always high enough to allow the efficient derivation of cells containing TALEN-driven mutations. Thus, simple methods to enrich cells containing TALEN-directed mutations would facilitate the use of TALENs. Here we describe the enrichment of such cells using surrogate episomal reporters coupled with flow cytometric sorting, magnetic separation, or hygromycin selection." @default.
• W2065602923 created "2016-06-24" @default.
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• W2065602923 date "2014-08-01" @default.
• W2065602923 modified "2023-10-16" @default.
• W2065602923 title "Enrichment of cells with TALEN-induced mutations using surrogate reporters" @default.
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• W2065602923 doi "https://doi.org/10.1016/j.ymeth.2014.04.012" @default.
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