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• W2065616900 abstract "A triple signal amplification strategy was designed for ultrasensitive immunosensing of cancer biomarker. This strategy was achieved using graphene to modify immunosensor surface for accelerating electron transfer, poly(styrene-co-acrylic acid) microbead (PSA) carried gold nanoparticles (AuNPs) as tracing tag to label signal antibody (Ab2) and AuNPs induced silver deposition for anodic stripping analysis. The immunosensor was constructed by covalently immobilizing capture antibody on chitosan/electrochemically reduced graphene oxide film modified glass carbon electrode. The in situ synthesis of AuNPs led to the loading of numerous AuNPs on PSA surface and convenient labeling of the tag to Ab2. With a sandwich-type immunoreaction, the AuNPs/PSA labeled Ab2 was captured on the surface of an immunosensor to further induce a silver deposition process. The electrochemical stripping signal of the deposited silver nanoparticles in KCl was used to monitor the immunoreaction. The triple signal amplification greatly enhanced the sensitivity for biomarker detection. The proposed method could detect carcinoembryonic antigen with a linear range of 0.5 pg mL–1 to 0.5 ng mL–1 and a detection limit down to 0.12 pg mL–1. The immunosensor exhibited good stability and acceptable reproducibility and accuracy, indicating potential applications in clinical diagnostics." @default.
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• W2065616900 date "2012-03-30" @default.
• W2065616900 modified "2023-10-16" @default.
• W2065616900 title "Triple Signal Amplification of Graphene Film, Polybead Carried Gold Nanoparticles as Tracing Tag and Silver Deposition for Ultrasensitive Electrochemical Immunosensing" @default.
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• W2065616900 doi "https://doi.org/10.1021/ac3001435" @default.
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