FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2065624563> ?p ?o ?g. }

• W2065624563 endingPage "311" @default.
• W2065624563 startingPage "301" @default.
• W2065624563 abstract "Abstract. Death through apoptosis is the main process by which aged cells that have lost their function are eliminated. Apoptotic cells are usually detected microscopically by changes in their morphology. However, determination of early apoptotic events is important for in vitro (and ex vivo ) studies. The main objective of the present study is to find the most sensitive method for apoptosis detection in human peripheral blood mononuclear cells (PBMCs) by comparing six different methods following five different means of immunological stimulation at 3 and 5 days. Each of six apoptosis quantification methods, except the trypan blue exclusion test, is a combination of two stains, one for the specific detection of apoptotic cells and the other for the unspecific detection of dead cells. Values for apoptosis and mortality were compared with a reference method. The choice of apoptosis detection method is more important following 3 days of stimulation than after 5 days of stimulation ( P = 2 × 10 −6 versus P = 1 × 10 −2 ). In contrast, we find mortality measurements following the different means of stimulation highly significant at both 3 and 5 days ( F 2.28 = 7.9, P = 1.4 × 10 −6 at 3 days and F 2.28 = 8.5, P = 4.5 × 10 −7 at 5 days). Variation as a result of the combination of specific PBMC stimulation and the method used to detect apoptosis is reduced considerably with time ( F 1.58 + 3.7, P + 3 × 10 −7 at 3 days to F = (1.58) = 0.97, P = 0.5 at 5 days). Based on Tukey's test, YO‐PRO‐1 is the most sensitive stain for apoptosis and, when combined with 7‐AAD, provides an accurate measure of apoptosis and mortality. In conclusion, we propose YO‐PRO‐1/7‐AAD as a new combination and low‐cost alternative for the sensitive detection of early apoptosis." @default.
• W2065624563 created "2016-06-24" @default.
• W2065624563 creator A5019318755 @default.
• W2065624563 creator A5049881239 @default.
• W2065624563 creator A5059530776 @default.
• W2065624563 creator A5066306496 @default.
• W2065624563 creator A5070779376 @default.
• W2065624563 creator A5084994054 @default.
• W2065624563 date "2005-10-01" @default.
• W2065624563 modified "2023-10-15" @default.
• W2065624563 title "Comparison of apoptosis and mortality measurements in peripheral blood mononuclear cells (PBMCs) using multiple methods" @default.
• W2065624563 cites W1543659825 @default.
• W2065624563 cites W185419764 @default.
• W2065624563 cites W1963684624 @default.
• W2065624563 cites W1965565653 @default.
• W2065624563 cites W1975414573 @default.
• W2065624563 cites W1989722723 @default.
• W2065624563 cites W1991344211 @default.
• W2065624563 cites W1997673739 @default.
• W2065624563 cites W2019123564 @default.
• W2065624563 cites W2019922960 @default.
• W2065624563 cites W2028402798 @default.
• W2065624563 cites W2032490341 @default.
• W2065624563 cites W2035551570 @default.
• W2065624563 cites W2036937264 @default.
• W2065624563 cites W2056230941 @default.
• W2065624563 cites W2056964795 @default.
• W2065624563 cites W2058108603 @default.
• W2065624563 cites W2061497413 @default.
• W2065624563 cites W2067038367 @default.
• W2065624563 cites W2113999599 @default.
• W2065624563 cites W2328267773 @default.
• W2065624563 cites W4237005310 @default.
• W2065624563 cites W4244428845 @default.
• W2065624563 doi "https://doi.org/10.1111/j.1365-2184.2005.00351.x" @default.
• W2065624563 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6496915" @default.
• W2065624563 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16202038" @default.
• W2065624563 hasPublicationYear "2005" @default.
• W2065624563 type Work @default.
• W2065624563 sameAs 2065624563 @default.
• W2065624563 citedByCount "34" @default.
• W2065624563 countsByYear W20656245632013 @default.
• W2065624563 countsByYear W20656245632016 @default.
• W2065624563 countsByYear W20656245632018 @default.
• W2065624563 countsByYear W20656245632019 @default.
• W2065624563 countsByYear W20656245632020 @default.
• W2065624563 countsByYear W20656245632021 @default.
• W2065624563 countsByYear W20656245632022 @default.
• W2065624563 crossrefType "journal-article" @default.
• W2065624563 hasAuthorship W2065624563A5019318755 @default.
• W2065624563 hasAuthorship W2065624563A5049881239 @default.
• W2065624563 hasAuthorship W2065624563A5059530776 @default.
• W2065624563 hasAuthorship W2065624563A5066306496 @default.
• W2065624563 hasAuthorship W2065624563A5070779376 @default.
• W2065624563 hasAuthorship W2065624563A5084994054 @default.
• W2065624563 hasBestOaLocation W20656245632 @default.
• W2065624563 hasConcept C134018914 @default.
• W2065624563 hasConcept C137061746 @default.
• W2065624563 hasConcept C150903083 @default.
• W2065624563 hasConcept C16685009 @default.
• W2065624563 hasConcept C190283241 @default.
• W2065624563 hasConcept C202751555 @default.
• W2065624563 hasConcept C203014093 @default.
• W2065624563 hasConcept C207001950 @default.
• W2065624563 hasConcept C24998067 @default.
• W2065624563 hasConcept C2780070996 @default.
• W2065624563 hasConcept C31573885 @default.
• W2065624563 hasConcept C55493867 @default.
• W2065624563 hasConcept C71924100 @default.
• W2065624563 hasConcept C86803240 @default.
• W2065624563 hasConceptScore W2065624563C134018914 @default.
• W2065624563 hasConceptScore W2065624563C137061746 @default.
• W2065624563 hasConceptScore W2065624563C150903083 @default.
• W2065624563 hasConceptScore W2065624563C16685009 @default.
• W2065624563 hasConceptScore W2065624563C190283241 @default.
• W2065624563 hasConceptScore W2065624563C202751555 @default.
• W2065624563 hasConceptScore W2065624563C203014093 @default.
• W2065624563 hasConceptScore W2065624563C207001950 @default.
• W2065624563 hasConceptScore W2065624563C24998067 @default.
• W2065624563 hasConceptScore W2065624563C2780070996 @default.
• W2065624563 hasConceptScore W2065624563C31573885 @default.
• W2065624563 hasConceptScore W2065624563C55493867 @default.
• W2065624563 hasConceptScore W2065624563C71924100 @default.
• W2065624563 hasConceptScore W2065624563C86803240 @default.
• W2065624563 hasIssue "5" @default.
• W2065624563 hasLocation W20656245631 @default.
• W2065624563 hasLocation W20656245632 @default.
• W2065624563 hasLocation W20656245633 @default.
• W2065624563 hasLocation W20656245634 @default.
• W2065624563 hasOpenAccess W2065624563 @default.
• W2065624563 hasPrimaryLocation W20656245631 @default.
• W2065624563 hasRelatedWork W1503573035 @default.
• W2065624563 hasRelatedWork W1538921910 @default.
• W2065624563 hasRelatedWork W1573342219 @default.
• W2065624563 hasRelatedWork W1958773460 @default.
• W2065624563 hasRelatedWork W1981828008 @default.
• W2065624563 hasRelatedWork W1987851970 @default.
• W2065624563 hasRelatedWork W2054755401 @default.

• next