Matches in SemOpenAlex for { <https://semopenalex.org/work/W2065628343> ?p ?o ?g. }
Showing items 1 to 84 of
84
with 100 items per page.
- W2065628343 endingPage "179" @default.
- W2065628343 startingPage "175" @default.
- W2065628343 abstract "A protocol is described for coupling of carrier-free iodine to protein sulfhydryl groups via fluorescein maleimide. 125I is first coupled to fluorescein maleimide in the presence of chloramine T. Iodination is stopped with sodium thiosulfate, and the iodine-substituted fluorescein maleimide is reacted with free cysteines of the protein. Excess label is then removed by gel-permeation chromatography. The procedure avoids exposition of the protein to oxidative conditions and does not require purification of the labeled carrier reagent. Suitability of the method for a given protein can be evaluated spectrophotometrically without employing radioactivity. It can be applied under denaturing conditions and may be particularly useful with mutant proteins carrying engineered single cysteine residues at sites that are not functionally critical." @default.
- W2065628343 created "2016-06-24" @default.
- W2065628343 creator A5017260947 @default.
- W2065628343 creator A5024331876 @default.
- W2065628343 creator A5032970473 @default.
- W2065628343 creator A5042300878 @default.
- W2065628343 date "1997-11-01" @default.
- W2065628343 modified "2023-10-18" @default.
- W2065628343 title "Cysteine-Specific Radioiodination of Proteins with Fluorescein Maleimide" @default.
- W2065628343 cites W1522405831 @default.
- W2065628343 cites W2005925629 @default.
- W2065628343 cites W2005986251 @default.
- W2065628343 cites W2006370393 @default.
- W2065628343 cites W2070412894 @default.
- W2065628343 cites W258376682 @default.
- W2065628343 cites W4251258711 @default.
- W2065628343 doi "https://doi.org/10.1006/abio.1997.2364" @default.
- W2065628343 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9367500" @default.
- W2065628343 hasPublicationYear "1997" @default.
- W2065628343 type Work @default.
- W2065628343 sameAs 2065628343 @default.
- W2065628343 citedByCount "8" @default.
- W2065628343 countsByYear W20656283432013 @default.
- W2065628343 countsByYear W20656283432015 @default.
- W2065628343 countsByYear W20656283432016 @default.
- W2065628343 countsByYear W20656283432018 @default.
- W2065628343 countsByYear W20656283432023 @default.
- W2065628343 crossrefType "journal-article" @default.
- W2065628343 hasAuthorship W2065628343A5017260947 @default.
- W2065628343 hasAuthorship W2065628343A5024331876 @default.
- W2065628343 hasAuthorship W2065628343A5032970473 @default.
- W2065628343 hasAuthorship W2065628343A5042300878 @default.
- W2065628343 hasConcept C121332964 @default.
- W2065628343 hasConcept C178790620 @default.
- W2065628343 hasConcept C181199279 @default.
- W2065628343 hasConcept C185592680 @default.
- W2065628343 hasConcept C2778015030 @default.
- W2065628343 hasConcept C2779201268 @default.
- W2065628343 hasConcept C2780105360 @default.
- W2065628343 hasConcept C2781324293 @default.
- W2065628343 hasConcept C40875361 @default.
- W2065628343 hasConcept C43617362 @default.
- W2065628343 hasConcept C539932907 @default.
- W2065628343 hasConcept C55493867 @default.
- W2065628343 hasConcept C62520636 @default.
- W2065628343 hasConcept C65973085 @default.
- W2065628343 hasConcept C91881484 @default.
- W2065628343 hasConceptScore W2065628343C121332964 @default.
- W2065628343 hasConceptScore W2065628343C178790620 @default.
- W2065628343 hasConceptScore W2065628343C181199279 @default.
- W2065628343 hasConceptScore W2065628343C185592680 @default.
- W2065628343 hasConceptScore W2065628343C2778015030 @default.
- W2065628343 hasConceptScore W2065628343C2779201268 @default.
- W2065628343 hasConceptScore W2065628343C2780105360 @default.
- W2065628343 hasConceptScore W2065628343C2781324293 @default.
- W2065628343 hasConceptScore W2065628343C40875361 @default.
- W2065628343 hasConceptScore W2065628343C43617362 @default.
- W2065628343 hasConceptScore W2065628343C539932907 @default.
- W2065628343 hasConceptScore W2065628343C55493867 @default.
- W2065628343 hasConceptScore W2065628343C62520636 @default.
- W2065628343 hasConceptScore W2065628343C65973085 @default.
- W2065628343 hasConceptScore W2065628343C91881484 @default.
- W2065628343 hasIssue "2" @default.
- W2065628343 hasLocation W20656283431 @default.
- W2065628343 hasLocation W20656283432 @default.
- W2065628343 hasOpenAccess W2065628343 @default.
- W2065628343 hasPrimaryLocation W20656283431 @default.
- W2065628343 hasRelatedWork W1567122180 @default.
- W2065628343 hasRelatedWork W1966675694 @default.
- W2065628343 hasRelatedWork W1983605596 @default.
- W2065628343 hasRelatedWork W2110021684 @default.
- W2065628343 hasRelatedWork W2152241281 @default.
- W2065628343 hasRelatedWork W2313130765 @default.
- W2065628343 hasRelatedWork W2313252952 @default.
- W2065628343 hasRelatedWork W2313488920 @default.
- W2065628343 hasRelatedWork W2322921742 @default.
- W2065628343 hasRelatedWork W4252578157 @default.
- W2065628343 hasVolume "253" @default.
- W2065628343 isParatext "false" @default.
- W2065628343 isRetracted "false" @default.
- W2065628343 magId "2065628343" @default.
- W2065628343 workType "article" @default.