Matches in SemOpenAlex for { <https://semopenalex.org/work/W2065636705> ?p ?o ?g. }
Showing items 1 to 79 of
79
with 100 items per page.
- W2065636705 endingPage "353" @default.
- W2065636705 startingPage "353" @default.
- W2065636705 abstract "Aim To evaluate the effect of transport media and storage temperatures on the survival of two root canal isolates, Prevotella oralis and Fusobacterium nucleatum over time. Methodology A standardised inoculum of each bacterium was prepared and transferred via sterile paper points into triplicate 1 mL volumes of transport media (Phosphate buffer saline[PBS]; Viability medium Göteberg [VMG III]; Reduced transport fluid [RTF]; RTF plus 20% glycerol; Commercial Dental Transport Medium [DTM] and Skimmed milk plus 20% glycerol) The samples were placed at room temperature (22 °C), 4 °C and -20 °C and the number of colony forming units (cfu) determined at 0 (base line), 1, 4 and 24 h after inoculation. The percentage of cells surviving in each storage medium at each time period was calculated using the number of viable cfu at 0 h as the baseline. Results All the tested media were capable of maintaining bacterial viability for up to 1 h regardless of the temperature. Storage at 4 °C in RTF after 4 h gave the best bacterial survival rate among the transport media used. Median percentage survival rates were 82% for P. oralis to 92% for F. nucleatum. In order to store samples for longer than 4 h before microbiological processing then storage at -20 °C, in skimmed milk medium was the most effective medium in assisting bacterial recovery with 75% and 82% survival for F. nucleatum and P. oralis, respectively while PBS was the least effective (0–3% survival). Conclusion Due to the loss of bacterial viability during sample storage and transport, samples should ideally be processed within the first hour after collection. However, if this is not possible, the results from this study suggest samples should either be: (i) placed in RTF and stored at 4 °C for no more than 4 h; or (ii) for overnight storage, samples should be placed in skimmed milk medium plus 20% glycerol at -20 °C." @default.
- W2065636705 created "2016-06-24" @default.
- W2065636705 creator A5002782178 @default.
- W2065636705 creator A5018319446 @default.
- W2065636705 creator A5035149734 @default.
- W2065636705 creator A5049322679 @default.
- W2065636705 creator A5085777724 @default.
- W2065636705 date "2010-04-01" @default.
- W2065636705 modified "2023-10-05" @default.
- W2065636705 title "Effect of transport and storage conditions on recovery of root canal isolates" @default.
- W2065636705 doi "https://doi.org/10.1111/j.1365-2591.2010.01682_8.x" @default.
- W2065636705 hasPublicationYear "2010" @default.
- W2065636705 type Work @default.
- W2065636705 sameAs 2065636705 @default.
- W2065636705 citedByCount "1" @default.
- W2065636705 countsByYear W20656367052016 @default.
- W2065636705 crossrefType "journal-article" @default.
- W2065636705 hasAuthorship W2065636705A5002782178 @default.
- W2065636705 hasAuthorship W2065636705A5018319446 @default.
- W2065636705 hasAuthorship W2065636705A5035149734 @default.
- W2065636705 hasAuthorship W2065636705A5049322679 @default.
- W2065636705 hasAuthorship W2065636705A5085777724 @default.
- W2065636705 hasConcept C140793950 @default.
- W2065636705 hasConcept C144027150 @default.
- W2065636705 hasConcept C180032290 @default.
- W2065636705 hasConcept C185592680 @default.
- W2065636705 hasConcept C189977493 @default.
- W2065636705 hasConcept C199343813 @default.
- W2065636705 hasConcept C2777349128 @default.
- W2065636705 hasConcept C2779747767 @default.
- W2065636705 hasConcept C2780183776 @default.
- W2065636705 hasConcept C2780881558 @default.
- W2065636705 hasConcept C31903555 @default.
- W2065636705 hasConcept C42972112 @default.
- W2065636705 hasConcept C523546767 @default.
- W2065636705 hasConcept C54355233 @default.
- W2065636705 hasConcept C55493867 @default.
- W2065636705 hasConcept C71924100 @default.
- W2065636705 hasConcept C86803240 @default.
- W2065636705 hasConcept C89423630 @default.
- W2065636705 hasConceptScore W2065636705C140793950 @default.
- W2065636705 hasConceptScore W2065636705C144027150 @default.
- W2065636705 hasConceptScore W2065636705C180032290 @default.
- W2065636705 hasConceptScore W2065636705C185592680 @default.
- W2065636705 hasConceptScore W2065636705C189977493 @default.
- W2065636705 hasConceptScore W2065636705C199343813 @default.
- W2065636705 hasConceptScore W2065636705C2777349128 @default.
- W2065636705 hasConceptScore W2065636705C2779747767 @default.
- W2065636705 hasConceptScore W2065636705C2780183776 @default.
- W2065636705 hasConceptScore W2065636705C2780881558 @default.
- W2065636705 hasConceptScore W2065636705C31903555 @default.
- W2065636705 hasConceptScore W2065636705C42972112 @default.
- W2065636705 hasConceptScore W2065636705C523546767 @default.
- W2065636705 hasConceptScore W2065636705C54355233 @default.
- W2065636705 hasConceptScore W2065636705C55493867 @default.
- W2065636705 hasConceptScore W2065636705C71924100 @default.
- W2065636705 hasConceptScore W2065636705C86803240 @default.
- W2065636705 hasConceptScore W2065636705C89423630 @default.
- W2065636705 hasIssue "4" @default.
- W2065636705 hasLocation W20656367051 @default.
- W2065636705 hasOpenAccess W2065636705 @default.
- W2065636705 hasPrimaryLocation W20656367051 @default.
- W2065636705 hasRelatedWork W1563035761 @default.
- W2065636705 hasRelatedWork W1908987995 @default.
- W2065636705 hasRelatedWork W1992304857 @default.
- W2065636705 hasRelatedWork W2035733734 @default.
- W2065636705 hasRelatedWork W2044380556 @default.
- W2065636705 hasRelatedWork W2048473055 @default.
- W2065636705 hasRelatedWork W2081686363 @default.
- W2065636705 hasRelatedWork W2138661975 @default.
- W2065636705 hasRelatedWork W3099633676 @default.
- W2065636705 hasRelatedWork W4313014464 @default.
- W2065636705 hasVolume "43" @default.
- W2065636705 isParatext "false" @default.
- W2065636705 isRetracted "false" @default.
- W2065636705 magId "2065636705" @default.
- W2065636705 workType "article" @default.