FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2065658857> ?p ?o ?g. }

• W2065658857 endingPage "522" @default.
• W2065658857 startingPage "517" @default.
• W2065658857 abstract "Inhibitors of anthrax lethal factor (LF) are currently being sought as effective therapeutics for the treatment of anthrax. Here we report a novel screening approach for inhibitors of LF, a yeast-hybrid-based assay system in which the expression of reporter genes from a Gal4 promoter is repressed by LF proteolytic activity. Yeast cells were co-transformed with LF and a chimeric transcription factor that contains an LF substrate sequence inserted between the DNA-binding and activation domains of Gal4. In the resulting yeast cells, LF cleaves the substrate, thus inactivating the chimeric Gal4 and resulting in lack of expression of reporter genes. Compounds that inhibit LF cleavage of its substrate are identified by changes in reporter gene activity. Relative to in vitro screens for inhibitors of LF proteolytic activity, this screen has the advantage of excluding compounds that are toxic or non-permeable to eukaryotic cells. Additionally, the screen has the advantage of being fast, easy and cheap because exogenous LF and substrate are not needed. An initial chemical library screen with this system has identified four candidate inhibitors which were confirmed to inhibit LF protease activity in an in vitro assay. Furthermore, FBS-00831, one of the compounds identified, protects Raw 264.7 macrophages from anthrax lethal toxin and the possible binding site on LF was also evaluated by molecular docking." @default.
• W2065658857 created "2016-06-24" @default.
• W2065658857 creator A5003956428 @default.
• W2065658857 creator A5004653425 @default.
• W2065658857 creator A5028287535 @default.
• W2065658857 creator A5050736930 @default.
• W2065658857 creator A5057041507 @default.
• W2065658857 creator A5070475503 @default.
• W2065658857 creator A5082985667 @default.
• W2065658857 date "2011-01-01" @default.
• W2065658857 modified "2023-09-25" @default.
• W2065658857 title "Yeast-hybrid based high-throughput assay for identification of anthrax lethal factor inhibitors" @default.
• W2065658857 cites W1966078827 @default.
• W2065658857 cites W1967383680 @default.
• W2065658857 cites W1968411426 @default.
• W2065658857 cites W1977186059 @default.
• W2065658857 cites W1991001144 @default.
• W2065658857 cites W2000206128 @default.
• W2065658857 cites W2006695188 @default.
• W2065658857 cites W2007234107 @default.
• W2065658857 cites W2009074190 @default.
• W2065658857 cites W2014719030 @default.
• W2065658857 cites W2033221350 @default.
• W2065658857 cites W2057895543 @default.
• W2065658857 cites W2075964433 @default.
• W2065658857 cites W2079326782 @default.
• W2065658857 cites W2088308362 @default.
• W2065658857 cites W2106177696 @default.
• W2065658857 cites W2107565679 @default.
• W2065658857 cites W2110325790 @default.
• W2065658857 cites W2143792029 @default.
• W2065658857 cites W2158534713 @default.
• W2065658857 cites W2175181799 @default.
• W2065658857 cites W2789853064 @default.
• W2065658857 cites W2949897611 @default.
• W2065658857 cites W4231530219 @default.
• W2065658857 cites W4242771440 @default.
• W2065658857 cites W4377077294 @default.
• W2065658857 cites W929520650 @default.
• W2065658857 doi "https://doi.org/10.1016/j.bbrc.2010.12.015" @default.
• W2065658857 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21144836" @default.
• W2065658857 hasPublicationYear "2011" @default.
• W2065658857 type Work @default.
• W2065658857 sameAs 2065658857 @default.
• W2065658857 citedByCount "2" @default.
• W2065658857 countsByYear W20656588572012 @default.
• W2065658857 countsByYear W20656588572016 @default.
• W2065658857 crossrefType "journal-article" @default.
• W2065658857 hasAuthorship W2065658857A5003956428 @default.
• W2065658857 hasAuthorship W2065658857A5004653425 @default.
• W2065658857 hasAuthorship W2065658857A5028287535 @default.
• W2065658857 hasAuthorship W2065658857A5050736930 @default.
• W2065658857 hasAuthorship W2065658857A5057041507 @default.
• W2065658857 hasAuthorship W2065658857A5070475503 @default.
• W2065658857 hasAuthorship W2065658857A5082985667 @default.
• W2065658857 hasConcept C104317684 @default.
• W2065658857 hasConcept C111829193 @default.
• W2065658857 hasConcept C123894998 @default.
• W2065658857 hasConcept C150194340 @default.
• W2065658857 hasConcept C151730666 @default.
• W2065658857 hasConcept C153911025 @default.
• W2065658857 hasConcept C161733203 @default.
• W2065658857 hasConcept C174851938 @default.
• W2065658857 hasConcept C175156509 @default.
• W2065658857 hasConcept C181199279 @default.
• W2065658857 hasConcept C185592680 @default.
• W2065658857 hasConcept C202751555 @default.
• W2065658857 hasConcept C2776714187 @default.
• W2065658857 hasConcept C2779222958 @default.
• W2065658857 hasConcept C40767141 @default.
• W2065658857 hasConcept C41810244 @default.
• W2065658857 hasConcept C43369102 @default.
• W2065658857 hasConcept C51323132 @default.
• W2065658857 hasConcept C55493867 @default.
• W2065658857 hasConcept C86339819 @default.
• W2065658857 hasConcept C86803240 @default.
• W2065658857 hasConceptScore W2065658857C104317684 @default.
• W2065658857 hasConceptScore W2065658857C111829193 @default.
• W2065658857 hasConceptScore W2065658857C123894998 @default.
• W2065658857 hasConceptScore W2065658857C150194340 @default.
• W2065658857 hasConceptScore W2065658857C151730666 @default.
• W2065658857 hasConceptScore W2065658857C153911025 @default.
• W2065658857 hasConceptScore W2065658857C161733203 @default.
• W2065658857 hasConceptScore W2065658857C174851938 @default.
• W2065658857 hasConceptScore W2065658857C175156509 @default.
• W2065658857 hasConceptScore W2065658857C181199279 @default.
• W2065658857 hasConceptScore W2065658857C185592680 @default.
• W2065658857 hasConceptScore W2065658857C202751555 @default.
• W2065658857 hasConceptScore W2065658857C2776714187 @default.
• W2065658857 hasConceptScore W2065658857C2779222958 @default.
• W2065658857 hasConceptScore W2065658857C40767141 @default.
• W2065658857 hasConceptScore W2065658857C41810244 @default.
• W2065658857 hasConceptScore W2065658857C43369102 @default.
• W2065658857 hasConceptScore W2065658857C51323132 @default.
• W2065658857 hasConceptScore W2065658857C55493867 @default.
• W2065658857 hasConceptScore W2065658857C86339819 @default.
• W2065658857 hasConceptScore W2065658857C86803240 @default.
• W2065658857 hasFunder F4320322035 @default.

• next