Matches in SemOpenAlex for { <https://semopenalex.org/work/W2065691102> ?p ?o ?g. }
Showing items 1 to 96 of
96
with 100 items per page.
- W2065691102 endingPage "11951" @default.
- W2065691102 startingPage "11944" @default.
- W2065691102 abstract "Optically detected magnetic resonance (ODMR) has been used to identify the binding site of a synthetic protamine subdomain to the major groove of DNA. A 14 amino acid peptide (R6WGR6) analog of the central DNA binding domain of bull protamine was synthesized with phenylalanine replaced by tryptophan (Trp). The peptide was bound to double-stranded poly(dABrdU) and to calf thymus DNA (CT DNA) and the complexes characterized as “wet” solids using ODMR techniques. The appearance of the D + E transition in the slow passage ODMR and of short-lived components in the phosphorescence decay of the complex of R6WGR6 with poly(dABrdU) is diagnostic of a heavy atom effect. This can only occur if the peptide binds in the major groove of poly(dABrdU). The microenvironment of Trp in the nucleoprotein complex was characterized by phosphorescence, radiative decay lifetimes, and low-temperature ODMR measurements before and after binding to DNA. Bathochromic shifts in the phosphorescence emission upon exciting to the red in CT DNA−peptide suggest that the Trp is in a polar environment, while the red-shifted position of the 0,0-band emission points to a more polarizable environment. The heavy atom effect strongly suggests a Trp location within the major groove of DNA. A partial stacking of Trp with the polarizable nucleobases and simultaneous interactions with the phosphate-guanidinium ion pairs and/or water molecules in the major groove of DNA which might not be totally displaced upon binding of the peptide could explain this conflicting evidence. Extrapolation of results from the system studied to protamine binding in sperm chromatin strongly suggests that the predominant binding site of protamine is the major groove of DNA." @default.
- W2065691102 created "2016-06-24" @default.
- W2065691102 creator A5010367025 @default.
- W2065691102 creator A5031883721 @default.
- W2065691102 creator A5049702210 @default.
- W2065691102 date "1997-09-01" @default.
- W2065691102 modified "2023-10-18" @default.
- W2065691102 title "Analysis of DNA−Protamine Interactions by Optical Detection of Magnetic Resonance" @default.
- W2065691102 cites W1563722950 @default.
- W2065691102 cites W1577332561 @default.
- W2065691102 cites W1967140358 @default.
- W2065691102 cites W1968787569 @default.
- W2065691102 cites W1982841657 @default.
- W2065691102 cites W2006997055 @default.
- W2065691102 cites W2019801529 @default.
- W2065691102 cites W2072697652 @default.
- W2065691102 cites W2080796053 @default.
- W2065691102 cites W2083009384 @default.
- W2065691102 cites W2094186244 @default.
- W2065691102 cites W2095288040 @default.
- W2065691102 cites W2127371436 @default.
- W2065691102 cites W2159921510 @default.
- W2065691102 cites W650972495 @default.
- W2065691102 doi "https://doi.org/10.1021/bi971061l" @default.
- W2065691102 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9305988" @default.
- W2065691102 hasPublicationYear "1997" @default.
- W2065691102 type Work @default.
- W2065691102 sameAs 2065691102 @default.
- W2065691102 citedByCount "47" @default.
- W2065691102 countsByYear W20656911022012 @default.
- W2065691102 countsByYear W20656911022013 @default.
- W2065691102 countsByYear W20656911022014 @default.
- W2065691102 countsByYear W20656911022015 @default.
- W2065691102 countsByYear W20656911022016 @default.
- W2065691102 countsByYear W20656911022017 @default.
- W2065691102 countsByYear W20656911022018 @default.
- W2065691102 countsByYear W20656911022020 @default.
- W2065691102 countsByYear W20656911022021 @default.
- W2065691102 countsByYear W20656911022022 @default.
- W2065691102 countsByYear W20656911022023 @default.
- W2065691102 crossrefType "journal-article" @default.
- W2065691102 hasAuthorship W2065691102A5010367025 @default.
- W2065691102 hasAuthorship W2065691102A5031883721 @default.
- W2065691102 hasAuthorship W2065691102A5049702210 @default.
- W2065691102 hasConcept C121332964 @default.
- W2065691102 hasConcept C12554922 @default.
- W2065691102 hasConcept C181817918 @default.
- W2065691102 hasConcept C185592680 @default.
- W2065691102 hasConcept C2776706248 @default.
- W2065691102 hasConcept C2779281246 @default.
- W2065691102 hasConcept C515207424 @default.
- W2065691102 hasConcept C552990157 @default.
- W2065691102 hasConcept C55493867 @default.
- W2065691102 hasConcept C61234307 @default.
- W2065691102 hasConcept C62520636 @default.
- W2065691102 hasConcept C71240020 @default.
- W2065691102 hasConcept C8010536 @default.
- W2065691102 hasConcept C86803240 @default.
- W2065691102 hasConcept C91881484 @default.
- W2065691102 hasConceptScore W2065691102C121332964 @default.
- W2065691102 hasConceptScore W2065691102C12554922 @default.
- W2065691102 hasConceptScore W2065691102C181817918 @default.
- W2065691102 hasConceptScore W2065691102C185592680 @default.
- W2065691102 hasConceptScore W2065691102C2776706248 @default.
- W2065691102 hasConceptScore W2065691102C2779281246 @default.
- W2065691102 hasConceptScore W2065691102C515207424 @default.
- W2065691102 hasConceptScore W2065691102C552990157 @default.
- W2065691102 hasConceptScore W2065691102C55493867 @default.
- W2065691102 hasConceptScore W2065691102C61234307 @default.
- W2065691102 hasConceptScore W2065691102C62520636 @default.
- W2065691102 hasConceptScore W2065691102C71240020 @default.
- W2065691102 hasConceptScore W2065691102C8010536 @default.
- W2065691102 hasConceptScore W2065691102C86803240 @default.
- W2065691102 hasConceptScore W2065691102C91881484 @default.
- W2065691102 hasIssue "39" @default.
- W2065691102 hasLocation W20656911021 @default.
- W2065691102 hasLocation W20656911022 @default.
- W2065691102 hasOpenAccess W2065691102 @default.
- W2065691102 hasPrimaryLocation W20656911021 @default.
- W2065691102 hasRelatedWork W1965579221 @default.
- W2065691102 hasRelatedWork W1968070835 @default.
- W2065691102 hasRelatedWork W2002922241 @default.
- W2065691102 hasRelatedWork W2009511063 @default.
- W2065691102 hasRelatedWork W2033358700 @default.
- W2065691102 hasRelatedWork W2044918281 @default.
- W2065691102 hasRelatedWork W2047637283 @default.
- W2065691102 hasRelatedWork W2069536711 @default.
- W2065691102 hasRelatedWork W4246319802 @default.
- W2065691102 hasRelatedWork W4323342626 @default.
- W2065691102 hasVolume "36" @default.
- W2065691102 isParatext "false" @default.
- W2065691102 isRetracted "false" @default.
- W2065691102 magId "2065691102" @default.
- W2065691102 workType "article" @default.