FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2065702518> ?p ?o ?g. }

• W2065702518 endingPage "213" @default.
• W2065702518 startingPage "199" @default.
• W2065702518 abstract "Inflamed tissues generate reactive nitrogen oxide species (RNO(x)), such as peroxynitrite (ONOO-)and nitryl chloride (NO2Cl), which lead to formation of nitrated DNA and protein adducts, including 8-nitroguanine (8NG), 8-nitroxanthine (8NX), and 3-nitrotyrosine (3NT). Once formed, the two nitrated DNA adducts are not stable in DNA and undergo spontaneous depurination. Nitration of protein tyrosine leads to inactivation of protein functions and 3NT has been detected in various disease states. We herein report that reduction of these nitro adducts to their corresponding amino analogues can be catalyzed by lipoyl dehydrogenases (EC 1.8.1.4) from Clostridium kluyveri (ck) and from porcine heart (ph) using NAD(P)H as the cofactor. We also found that dihydrolipoic acid (DHLA) and ubiquinol can be used as effective cofactors for reduction of 8NG, 8NX, and 3NT by these lipoyl dehydrogenases. The reduction efficiency of the mammalian enzyme is higher than the bacterial isozyme. The preference of cofactors by both lipoyl dehydrogenases is DHLA>NAD(P)H>ubiquinol. In all the systems examined, the nitrated purines are reduced to a greater extent than 3NT under the same conditions. We also demonstrate that this lipoyl dehydrogenase/antioxidant system is effective in reducing nitrated purine on NO2Cl-treated double stranded calf thymus DNA, and thus decreases apurinic site formation. The nitroreductase activity for lipoyl dehydrogenase might represent a possible metabolic pathway to reverse the process of biological nitration." @default.
• W2065702518 created "2016-06-24" @default.
• W2065702518 creator A5023265257 @default.
• W2065702518 creator A5049448823 @default.
• W2065702518 creator A5055868375 @default.
• W2065702518 date "2002-08-01" @default.
• W2065702518 modified "2023-10-16" @default.
• W2065702518 title "Lipoyl dehydrogenase catalyzes reduction of nitrated DNA and protein adducts using dihydrolipoic acid or ubiquinol as the cofactor" @default.
• W2065702518 cites W1489860413 @default.
• W2065702518 cites W1598255119 @default.
• W2065702518 cites W1725300350 @default.
• W2065702518 cites W1727895137 @default.
• W2065702518 cites W1749950256 @default.
• W2065702518 cites W1819471509 @default.
• W2065702518 cites W1965696818 @default.
• W2065702518 cites W1967315269 @default.
• W2065702518 cites W1969880500 @default.
• W2065702518 cites W1975515113 @default.
• W2065702518 cites W1976178228 @default.
• W2065702518 cites W1978601789 @default.
• W2065702518 cites W1979230311 @default.
• W2065702518 cites W1981502718 @default.
• W2065702518 cites W1981759285 @default.
• W2065702518 cites W1983400389 @default.
• W2065702518 cites W1999366850 @default.
• W2065702518 cites W1999649376 @default.
• W2065702518 cites W2001565942 @default.
• W2065702518 cites W2006022248 @default.
• W2065702518 cites W2006479775 @default.
• W2065702518 cites W2007409492 @default.
• W2065702518 cites W2011643270 @default.
• W2065702518 cites W2018740639 @default.
• W2065702518 cites W2018748912 @default.
• W2065702518 cites W2024379653 @default.
• W2065702518 cites W2035464530 @default.
• W2065702518 cites W2038443620 @default.
• W2065702518 cites W2046244465 @default.
• W2065702518 cites W2047936141 @default.
• W2065702518 cites W2052350481 @default.
• W2065702518 cites W2055074116 @default.
• W2065702518 cites W2058053999 @default.
• W2065702518 cites W2058137293 @default.
• W2065702518 cites W2065949544 @default.
• W2065702518 cites W2069091687 @default.
• W2065702518 cites W2076824965 @default.
• W2065702518 cites W2083259478 @default.
• W2065702518 cites W2089796673 @default.
• W2065702518 cites W2092094594 @default.
• W2065702518 cites W2092967940 @default.
• W2065702518 cites W2094050302 @default.
• W2065702518 cites W2094213529 @default.
• W2065702518 cites W2094471857 @default.
• W2065702518 cites W2094745968 @default.
• W2065702518 cites W2097811288 @default.
• W2065702518 cites W2111415702 @default.
• W2065702518 cites W2116429930 @default.
• W2065702518 cites W2128022125 @default.
• W2065702518 cites W2133615399 @default.
• W2065702518 cites W2134386806 @default.
• W2065702518 cites W2137672734 @default.
• W2065702518 cites W2140857653 @default.
• W2065702518 cites W2150882549 @default.
• W2065702518 cites W2166887613 @default.
• W2065702518 cites W2170104499 @default.
• W2065702518 cites W2336238528 @default.
• W2065702518 cites W2337633326 @default.
• W2065702518 cites W3026468109 @default.
• W2065702518 cites W4238552834 @default.
• W2065702518 doi "https://doi.org/10.1016/s0009-2797(02)00019-4" @default.
• W2065702518 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12204577" @default.
• W2065702518 hasPublicationYear "2002" @default.
• W2065702518 type Work @default.
• W2065702518 sameAs 2065702518 @default.
• W2065702518 citedByCount "22" @default.
• W2065702518 countsByYear W20657025182012 @default.
• W2065702518 countsByYear W20657025182013 @default.
• W2065702518 countsByYear W20657025182017 @default.
• W2065702518 countsByYear W20657025182018 @default.
• W2065702518 countsByYear W20657025182019 @default.
• W2065702518 countsByYear W20657025182022 @default.
• W2065702518 countsByYear W20657025182023 @default.
• W2065702518 crossrefType "journal-article" @default.
• W2065702518 hasAuthorship W2065702518A5023265257 @default.
• W2065702518 hasAuthorship W2065702518A5049448823 @default.
• W2065702518 hasAuthorship W2065702518A5055868375 @default.
• W2065702518 hasConcept C178790620 @default.
• W2065702518 hasConcept C181199279 @default.
• W2065702518 hasConcept C185592680 @default.
• W2065702518 hasConcept C197957613 @default.
• W2065702518 hasConcept C2775856698 @default.
• W2065702518 hasConcept C2777337673 @default.
• W2065702518 hasConcept C2778784160 @default.
• W2065702518 hasConcept C2780795997 @default.
• W2065702518 hasConcept C34737976 @default.
• W2065702518 hasConcept C552990157 @default.
• W2065702518 hasConcept C55493867 @default.
• W2065702518 hasConcept C71240020 @default.
• W2065702518 hasConcept C75520062 @default.

• next