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- W2065755318 abstract "Two amylases of an alkalopsychrotrophic Micrococcus were purified by chromatographies of DEAE-Toyopearl, Butyl-Toyopearl and Shodex WS-2003. Molecular weights and pI values of the purified enzymes, I and II, were 185000 and 125000 by SDS-PAGE and 4.8 and 4.3 by isoelectric focusing, respectively. Enzyme I had not only amylase but also pullulanase activity. In the presence of Ca2+ ions, other properties of both enzymes were very similar: optimum temperature 55–60°C, optimum pH 7.5–8.0 km value for maltopentaose 0.09 mM. Both amylases were completely inactivated after incubation with EDTA at 30°C and thereafter, could be reactivated by an addition of CaCl2. In the absence of Ca2+ ions, amylase I became thermoresistant, while the thermostability of amylase II decreased. Neither amylase activity of enzyme I nor enzyme II was inhibited by pullulan. Reinigung und Charakterisierung der α-Amylasen aus einem alkalipsychrotrophen Micrococcus sp. Zwei Amylasen eines alkalopsychrotropen Micrococcus wurden durch Chromatographie über DEAE-Toyopearl, Butyl-Toyopearl und Shodex WS-2003 gereinigt. Die Molekulargewichte und pI-Werte der gereinigten Enzyme I und II betrugen 185000 und 125000 mittels SDS-PAGE bzw. 4,8 und 4,3 mittels isoelektrischer Focussierung. Das Enzym I hatte nicht nur Amylase-, sondern auch Pullulanaseaktivität. In Gegenwart von Ca2+-Ionen waren sich andere Eigenschaften der beiden Enzyme sehr ähnlich: Temperaturoptimum 55–60°C und pH-Optimum 7,5–8,0; die Km für Maltopentaose betrug 0,09 mM. Beide Amylasen waren nach Inkubation mit EDTA bei 30°C vollständig inaktiviert, konnten jedoch danach durch Zusatz von CaCl2 reaktiviert werden. Bei Abwesenheit von Ca2+-Ionen wurde Amylase I thermoresistent, während sich die Thermostabilität der Amylase II verringerte. Die Amylaseaktivitäten der Enzyme I und II wurden durch Pullulan nicht inhibiert." @default.
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- W2065755318 date "1990-01-01" @default.
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- W2065755318 title "Purification and Characterization of α-Amylases of an AlkalopsychrotrophicMicrococcus sp" @default.
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- W2065755318 doi "https://doi.org/10.1002/star.19900421008" @default.
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