Matches in SemOpenAlex for { <https://semopenalex.org/work/W2065757981> ?p ?o ?g. }
- W2065757981 endingPage "2601" @default.
- W2065757981 startingPage "2597" @default.
- W2065757981 abstract "The analysis of intact glycopeptides by mass spectrometry is challenging due to the numerous possibilities for isomerization, both within the attached glycan and the location of the modification on the peptide backbone. Here, we demonstrate that high field asymmetric wave ion mobility spectrometry (FAIMS), also known as differential ion mobility, is able to separate isomeric O-linked glycopeptides that have identical sequences but differing sites of glycosylation. Two glycopeptides from the glycoprotein mucin 5AC, GT(GalNAc)TPSPVPTTSTTSAP and GTTPSPVPTTST(GalNAc)TSAP (where GalNAc is O-linked N-acetylgalactosamine), were shown to coelute following reversed-phase liquid chromatography. However, FAIMS analysis of the glycopeptides revealed that the compensation voltage ranges in which the peptides were transmitted differed. Thus, it is possible at certain compensation voltages to completely separate the glycopeptides. Separation of the glycopeptides was confirmed by unique reporter ions produced by supplemental activation electron transfer dissociation mass spectrometry. These fragments also enable localization of the site of glycosylation. The results suggest that glycan position plays a key role in determining gas-phase glycopeptide structure and have implications for the application of FAIMS in glycoproteomics." @default.
- W2065757981 created "2016-06-24" @default.
- W2065757981 creator A5014883632 @default.
- W2065757981 creator A5078797993 @default.
- W2065757981 date "2012-02-10" @default.
- W2065757981 modified "2023-10-17" @default.
- W2065757981 title "Separation and Identification of Isomeric Glycopeptides by High Field Asymmetric Waveform Ion Mobility Spectrometry" @default.
- W2065757981 cites W1605996579 @default.
- W2065757981 cites W190025346 @default.
- W2065757981 cites W1970261233 @default.
- W2065757981 cites W1980427396 @default.
- W2065757981 cites W1984370122 @default.
- W2065757981 cites W1985625499 @default.
- W2065757981 cites W2003250682 @default.
- W2065757981 cites W2004125187 @default.
- W2065757981 cites W2010927211 @default.
- W2065757981 cites W2013313370 @default.
- W2065757981 cites W2014419786 @default.
- W2065757981 cites W2020266605 @default.
- W2065757981 cites W2024191848 @default.
- W2065757981 cites W2041051086 @default.
- W2065757981 cites W2049369177 @default.
- W2065757981 cites W2056075909 @default.
- W2065757981 cites W2059556669 @default.
- W2065757981 cites W2062236151 @default.
- W2065757981 cites W2062429736 @default.
- W2065757981 cites W2062727375 @default.
- W2065757981 cites W2071489119 @default.
- W2065757981 cites W2077277234 @default.
- W2065757981 cites W2078125368 @default.
- W2065757981 cites W2079115619 @default.
- W2065757981 cites W2080487519 @default.
- W2065757981 cites W2112947615 @default.
- W2065757981 cites W2124551362 @default.
- W2065757981 cites W2129232972 @default.
- W2065757981 cites W2134276538 @default.
- W2065757981 cites W2155482447 @default.
- W2065757981 cites W4244582831 @default.
- W2065757981 doi "https://doi.org/10.1021/ac203321y" @default.
- W2065757981 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3295202" @default.
- W2065757981 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22280549" @default.
- W2065757981 hasPublicationYear "2012" @default.
- W2065757981 type Work @default.
- W2065757981 sameAs 2065757981 @default.
- W2065757981 citedByCount "100" @default.
- W2065757981 countsByYear W20657579812012 @default.
- W2065757981 countsByYear W20657579812013 @default.
- W2065757981 countsByYear W20657579812014 @default.
- W2065757981 countsByYear W20657579812015 @default.
- W2065757981 countsByYear W20657579812016 @default.
- W2065757981 countsByYear W20657579812017 @default.
- W2065757981 countsByYear W20657579812018 @default.
- W2065757981 countsByYear W20657579812019 @default.
- W2065757981 countsByYear W20657579812020 @default.
- W2065757981 countsByYear W20657579812021 @default.
- W2065757981 countsByYear W20657579812022 @default.
- W2065757981 countsByYear W20657579812023 @default.
- W2065757981 crossrefType "journal-article" @default.
- W2065757981 hasAuthorship W2065757981A5014883632 @default.
- W2065757981 hasAuthorship W2065757981A5078797993 @default.
- W2065757981 hasBestOaLocation W20657579811 @default.
- W2065757981 hasConcept C108625454 @default.
- W2065757981 hasConcept C137610392 @default.
- W2065757981 hasConcept C15213710 @default.
- W2065757981 hasConcept C156534840 @default.
- W2065757981 hasConcept C162356407 @default.
- W2065757981 hasConcept C185592680 @default.
- W2065757981 hasConcept C206212055 @default.
- W2065757981 hasConcept C2777109674 @default.
- W2065757981 hasConcept C2777313579 @default.
- W2065757981 hasConcept C2779281246 @default.
- W2065757981 hasConcept C31827203 @default.
- W2065757981 hasConcept C43617362 @default.
- W2065757981 hasConcept C501593827 @default.
- W2065757981 hasConcept C55493867 @default.
- W2065757981 hasConceptScore W2065757981C108625454 @default.
- W2065757981 hasConceptScore W2065757981C137610392 @default.
- W2065757981 hasConceptScore W2065757981C15213710 @default.
- W2065757981 hasConceptScore W2065757981C156534840 @default.
- W2065757981 hasConceptScore W2065757981C162356407 @default.
- W2065757981 hasConceptScore W2065757981C185592680 @default.
- W2065757981 hasConceptScore W2065757981C206212055 @default.
- W2065757981 hasConceptScore W2065757981C2777109674 @default.
- W2065757981 hasConceptScore W2065757981C2777313579 @default.
- W2065757981 hasConceptScore W2065757981C2779281246 @default.
- W2065757981 hasConceptScore W2065757981C31827203 @default.
- W2065757981 hasConceptScore W2065757981C43617362 @default.
- W2065757981 hasConceptScore W2065757981C501593827 @default.
- W2065757981 hasConceptScore W2065757981C55493867 @default.
- W2065757981 hasIssue "5" @default.
- W2065757981 hasLocation W20657579811 @default.
- W2065757981 hasLocation W20657579812 @default.
- W2065757981 hasLocation W20657579813 @default.
- W2065757981 hasLocation W20657579814 @default.
- W2065757981 hasOpenAccess W2065757981 @default.
- W2065757981 hasPrimaryLocation W20657579811 @default.
- W2065757981 hasRelatedWork W2088770711 @default.
- W2065757981 hasRelatedWork W2529856847 @default.