FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2065767568> ?p ?o ?g. }

• W2065767568 endingPage "4297" @default.
• W2065767568 startingPage "4288" @default.
• W2065767568 abstract "The role of N-linked glycosylation of beta-subunits in the functional properties of the oligomeric P-type ATPases Na,K- and H,K-ATPase has been examined by expressing glycosylation-deficient Asn-to-Gln beta-variants in Xenopus oocytes. For both ATPases, the absence of the huge N-linked oligosaccharide moiety on the beta-subunit does not affect alpha/beta coassembly, plasma membrane delivery or functional activity of the holoenzyme. Whereas this is in line with several previous glycosylation studies on Na,K-ATPase, this is the first report showing that the cell surface delivery and enzymatic activity of the gastric H,K-ATPase is unaffected by the lack of N-linked glycosylation. Sulfhydryl-specific labeling of introduced cysteine reporter sites with the environmentally sensitive fluorophore tetramethylrhodamine-6-maleimide (TMRM) upon expression in Xenopus oocytes enabled us to further investigate potential effects of the N-glycans on more subtle enzymatic properties, like the distribution between E 1P/E 2P states of the catalytic cycle and the kinetics of the E 1P/E 2P conformational transition under presteady state conditions. For both Na,K-ATPase and H,K-ATPase, we observed differences in neither the voltage-dependent E 1P/E 2P ratio nor the kinetics of the E 1P/E 2P transition between holoenzymes comprising glycosylated and glycosylation-deficient beta-subunits. We conclude that the N-linked glycans on these essential accessory subunits of oligomeric P-type ATPases are dispensable for proper folding, membrane stabilization of the alpha-subunit and transport function itself. Glycosylation is rather important for other cellular functions not relevant in the oocyte expression system, such as intercellular interactions or basolateral versus apical targeting in polarized cells, as demonstrated in other expression systems." @default.
• W2065767568 created "2016-06-24" @default.
• W2065767568 creator A5005757182 @default.
• W2065767568 creator A5030302118 @default.
• W2065767568 creator A5066662505 @default.
• W2065767568 creator A5069837459 @default.
• W2065767568 creator A5090635713 @default.
• W2065767568 date "2008-03-15" @default.
• W2065767568 modified "2023-10-03" @default.
• W2065767568 title "Characterization of Na,K-ATPase and H,K-ATPase Enzymes with Glycosylation-Deficient β-Subunit Variants by Voltage-Clamp Fluorometry in <i>Xenopus</i> Oocytes" @default.
• W2065767568 cites W1532133113 @default.
• W2065767568 cites W1562290896 @default.
• W2065767568 cites W1604175695 @default.
• W2065767568 cites W1647423442 @default.
• W2065767568 cites W1689972459 @default.
• W2065767568 cites W1967604793 @default.
• W2065767568 cites W1968082615 @default.
• W2065767568 cites W1968922132 @default.
• W2065767568 cites W1973623730 @default.
• W2065767568 cites W1977034717 @default.
• W2065767568 cites W1977306386 @default.
• W2065767568 cites W1984370122 @default.
• W2065767568 cites W1986094136 @default.
• W2065767568 cites W1990188756 @default.
• W2065767568 cites W1993693324 @default.
• W2065767568 cites W2010102263 @default.
• W2065767568 cites W2011497636 @default.
• W2065767568 cites W2014538976 @default.
• W2065767568 cites W2021194016 @default.
• W2065767568 cites W2022903648 @default.
• W2065767568 cites W2024327957 @default.
• W2065767568 cites W2027409743 @default.
• W2065767568 cites W2039344808 @default.
• W2065767568 cites W2046714822 @default.
• W2065767568 cites W2053533928 @default.
• W2065767568 cites W2054385136 @default.
• W2065767568 cites W2063395138 @default.
• W2065767568 cites W2063806420 @default.
• W2065767568 cites W2064036102 @default.
• W2065767568 cites W2064872693 @default.
• W2065767568 cites W2067999317 @default.
• W2065767568 cites W2070012573 @default.
• W2065767568 cites W2071238400 @default.
• W2065767568 cites W2073077068 @default.
• W2065767568 cites W2078399257 @default.
• W2065767568 cites W2079357395 @default.
• W2065767568 cites W2100837269 @default.
• W2065767568 cites W2103161330 @default.
• W2065767568 cites W2112487257 @default.
• W2065767568 cites W2116128630 @default.
• W2065767568 cites W2117875146 @default.
• W2065767568 cites W2152516606 @default.
• W2065767568 cites W2155523215 @default.
• W2065767568 cites W2167355761 @default.
• W2065767568 cites W2256761341 @default.
• W2065767568 cites W2307595411 @default.
• W2065767568 cites W2342978219 @default.
• W2065767568 cites W2412808135 @default.
• W2065767568 doi "https://doi.org/10.1021/bi800092k" @default.
• W2065767568 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18341291" @default.
• W2065767568 hasPublicationYear "2008" @default.
• W2065767568 type Work @default.
• W2065767568 sameAs 2065767568 @default.
• W2065767568 citedByCount "19" @default.
• W2065767568 countsByYear W20657675682012 @default.
• W2065767568 countsByYear W20657675682013 @default.
• W2065767568 countsByYear W20657675682015 @default.
• W2065767568 countsByYear W20657675682016 @default.
• W2065767568 countsByYear W20657675682019 @default.
• W2065767568 countsByYear W20657675682020 @default.
• W2065767568 countsByYear W20657675682021 @default.
• W2065767568 crossrefType "journal-article" @default.
• W2065767568 hasAuthorship W2065767568A5005757182 @default.
• W2065767568 hasAuthorship W2065767568A5030302118 @default.
• W2065767568 hasAuthorship W2065767568A5066662505 @default.
• W2065767568 hasAuthorship W2065767568A5069837459 @default.
• W2065767568 hasAuthorship W2065767568A5090635713 @default.
• W2065767568 hasConcept C104292427 @default.
• W2065767568 hasConcept C104317684 @default.
• W2065767568 hasConcept C12554922 @default.
• W2065767568 hasConcept C181199279 @default.
• W2065767568 hasConcept C185592680 @default.
• W2065767568 hasConcept C23265538 @default.
• W2065767568 hasConcept C2777313579 @default.
• W2065767568 hasConcept C2779535977 @default.
• W2065767568 hasConcept C2910882050 @default.
• W2065767568 hasConcept C2911069308 @default.
• W2065767568 hasConcept C55493867 @default.
• W2065767568 hasConcept C86803240 @default.
• W2065767568 hasConceptScore W2065767568C104292427 @default.
• W2065767568 hasConceptScore W2065767568C104317684 @default.
• W2065767568 hasConceptScore W2065767568C12554922 @default.
• W2065767568 hasConceptScore W2065767568C181199279 @default.
• W2065767568 hasConceptScore W2065767568C185592680 @default.
• W2065767568 hasConceptScore W2065767568C23265538 @default.
• W2065767568 hasConceptScore W2065767568C2777313579 @default.
• W2065767568 hasConceptScore W2065767568C2779535977 @default.
• W2065767568 hasConceptScore W2065767568C2910882050 @default.

• next