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W2065790861 abstract "Monocytes of healthy donors were infected with HIV1 in vitro: 14-21 days after infection 50-70% of the cells produced p24 HIV1 antigen as detected with anti-p24 immunostaining; infected cultures showed enhanced secretion of interleukin-6 (IL6), interleukin-8 (IL8) and tumour necrosis factor alpha (TNF-alpha). The expression of cytokines on the single-cell level was further analysed by in situ hybridization using nonradioactive digoxigenin for detection. HIV1 (p24+) -producing cells were compared with non-HIV (p24-) -producing cells. All morphological subtypes of macrophages showed HIV production; no difference in cytokine expression was observed. Immunocytochemistry of HIV-infected and uninfected cultures also showed no difference in the pattern of IL1-beta, IL6, IL8 and TNF-alpha protein expression in the cells." @default.
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W2065790861 date "1994-01-01" @default.
W2065790861 modified "2023-10-17" @default.
W2065790861 title "Cytokine expression of HIV-infected monocytes/macrophages at the single-cell level" @default.
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W2065790861 doi "https://doi.org/10.1016/s0923-2516(07)80022-7" @default.
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