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- W2065863039 abstract "The N-terminal region of the picornaviral 2C protein is predicted to fold into an amphipathic alpha-helix that is responsible for the protein's association with membranes in the viral RNA replication complex. We have identified a similar sequence in the N-terminal region of NS5A of hepaciviruses that was recently shown to form an amphipathic alpha-helix. The conservation of the N-terminal region in two apparently unrelated proteins of two different RNA virus families suggested that this helix might represent an independent module. To test this hypothesis, we constructed chimeric poliovirus (PV) genomes in which the sequence encoding the N-terminal 2C amphipathic helix was replaced by orthologous sequences from other picornaviral genomes or a similar sequence from NS5A of HCV. Effects of the mutations were assessed by measuring the accumulation of viable virus and viral RNA in HeLa cells after transfection, examining membrane morphology in cells expressing chimeric proteins and by in vitro analysis of RNA translation, protein processing and negative strand RNA synthesis in HeLa cell extracts. The chimeras manifested a wide range of growth and RNA synthesis phenotypes. The results are compatible with our hypothesis, although they demonstrate that helix exchangeability may be restricted due to requirements for interactions with other viral components involved in virus replication." @default.
- W2065863039 created "2016-06-24" @default.
- W2065863039 creator A5002796839 @default.
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- W2065863039 creator A5047488691 @default.
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- W2065863039 date "2006-01-01" @default.
- W2065863039 modified "2023-10-14" @default.
- W2065863039 title "Testing the modularity of the N-terminal amphipathic helix conserved in picornavirus 2C proteins and hepatitis C NS5A protein" @default.
- W2065863039 cites W1491556421 @default.
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- W2065863039 cites W1840105038 @default.
- W2065863039 cites W1916164841 @default.
- W2065863039 cites W1957657705 @default.
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- W2065863039 cites W1964468772 @default.
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- W2065863039 cites W1970999032 @default.
- W2065863039 cites W1974750479 @default.
- W2065863039 cites W1978512485 @default.
- W2065863039 cites W1980651773 @default.
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- W2065863039 cites W2015990311 @default.
- W2065863039 cites W2022125636 @default.
- W2065863039 cites W2024350223 @default.
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- W2065863039 cites W2033125798 @default.
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- W2065863039 cites W2048821073 @default.
- W2065863039 cites W2050524167 @default.
- W2065863039 cites W2050594447 @default.
- W2065863039 cites W2055043387 @default.
- W2065863039 cites W2056307424 @default.
- W2065863039 cites W2059530064 @default.
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- W2065863039 cites W2071755568 @default.
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- W2065863039 cites W2128957211 @default.
- W2065863039 cites W2132444008 @default.
- W2065863039 cites W2137110694 @default.
- W2065863039 cites W2139609944 @default.
- W2065863039 cites W2142182685 @default.
- W2065863039 cites W2142277942 @default.
- W2065863039 cites W2144538210 @default.
- W2065863039 cites W2148544414 @default.
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- W2065863039 cites W2150693868 @default.
- W2065863039 cites W2153142359 @default.
- W2065863039 cites W2161003987 @default.
- W2065863039 cites W2168079335 @default.
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- W2065863039 doi "https://doi.org/10.1016/j.virol.2005.08.044" @default.
- W2065863039 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7111807" @default.
- W2065863039 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16226781" @default.
- W2065863039 hasPublicationYear "2006" @default.
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