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- W2065883344 abstract "The gacA gene of the biocontrol strain Pseudomonas fluorescens CHA0 codes for a response regulator which, together with the sensor kinase GacS (=LemA), is required for the production of exoenzymes and secondary metabolites involved in biocontrol, including hydrogen cyanide (HCN). A gacA multicopy suppressor was isolated from a cosmid library of strain CHA0 and identified as the infC-rpmI-rplT operon, which encodes the translation initiation factor IF3 and the ribosomal proteins L35 and L20. The efficiency of suppression was about 30%, as determined by the use of a GacA-controlled reporter construct, i.e. a translational hcnA'-'lacZ fusion. Overexpression of the rsmA gene (coding for a global translational repressor) reversed the suppressive effect of the amplified infC operon. This finding suggests that some product(s) of the infC operon can compete with RsmA at the level of translation in P. fluorescens CHA0 and that important biocontrol traits can be regulated at this level." @default.
- W2065883344 created "2016-06-24" @default.
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- W2065883344 date "2000-06-01" @default.
- W2065883344 modified "2023-09-23" @default.
- W2065883344 title "Multicopy suppression of agacAmutation by theinfCoperon inPseudomonas fluorescensCHA0: competition with the global translational regulator RsmA" @default.
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- W2065883344 doi "https://doi.org/10.1111/j.1574-6968.2000.tb09136.x" @default.
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