FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2065890712> ?p ?o ?g. }

• W2065890712 endingPage "316" @default.
• W2065890712 startingPage "303" @default.
• W2065890712 abstract "Detection of pathogenic microorganisms in environmental samples is a difficult process. Concentration of the organisms of interest also co-concentrates inhibitors of many end-point detection methods, notably, nucleic acid methods. In addition, sensitive, highly multiplexed pathogen detection continues to be problematic. The primary function of the BEADS (Biodetection Enabling Analyte Delivery System) platform is the automated concentration and purification of target analytes from interfering substances, often present in these samples, via a renewable surface column. In one version of BEADS, automated immunomagnetic separation (IMS) is used to separate cells from their samples. Captured cells are transferred to a flow-through thermal cycler where PCR, using labeled primers, is performed. PCR products are then detected by hybridization to a DNA suspension array. In another version of BEADS, cell lysis is performed, and community RNA is purified and directly labeled. Multiplexed detection is accomplished by direct hybridization of the RNA to a planar microarray. The integrated IMS/PCR version of BEADS can successfully purify and amplify 10 E. coli O157:H7 cells from river water samples. Multiplexed PCR assays for the simultaneous detection of E. coli O157:H7, Salmonella, and Shigella on bead suspension arrays was demonstrated for the detection of as few as 100 cells for each organism. Results for the RNA version of BEADS are also showing promising results. Automation yields highly purified RNA, suitable for multiplexed detection on microarrays, with microarray detection specificity equivalent to PCR. Both versions of the BEADS platform show great promise for automated pathogen detection from environmental samples. Highly multiplexed pathogen detection using PCR continues to be problematic, but may be required for trace detection in large volume samples. The RNA approach solves the issues of highly multiplexed PCR and provides live vs. dead capabilities. However, sensitivity of the method will need to be improved for RNA analysis to replace PCR." @default.
• W2065890712 created "2016-06-24" @default.
• W2065890712 creator A5016193487 @default.
• W2065890712 creator A5022220145 @default.
• W2065890712 creator A5037566826 @default.
• W2065890712 creator A5037897736 @default.
• W2065890712 creator A5057904055 @default.
• W2065890712 creator A5086271534 @default.
• W2065890712 creator A5086936251 @default.
• W2065890712 creator A5087963967 @default.
• W2065890712 date "2005-09-01" @default.
• W2065890712 modified "2023-09-26" @default.
• W2065890712 title "Automated methods for multiplexed pathogen detection" @default.
• W2065890712 cites W1497810769 @default.
• W2065890712 cites W1510168356 @default.
• W2065890712 cites W1971635124 @default.
• W2065890712 cites W1975710631 @default.
• W2065890712 cites W1982090203 @default.
• W2065890712 cites W1984391109 @default.
• W2065890712 cites W1985080632 @default.
• W2065890712 cites W1985996007 @default.
• W2065890712 cites W1987994221 @default.
• W2065890712 cites W1988345904 @default.
• W2065890712 cites W2051051460 @default.
• W2065890712 cites W2055215158 @default.
• W2065890712 cites W2055800251 @default.
• W2065890712 cites W2062880911 @default.
• W2065890712 cites W2076211220 @default.
• W2065890712 cites W2093433979 @default.
• W2065890712 cites W2094818197 @default.
• W2065890712 cites W2101253259 @default.
• W2065890712 cites W2102821791 @default.
• W2065890712 cites W2104928583 @default.
• W2065890712 cites W2109928552 @default.
• W2065890712 cites W2115441357 @default.
• W2065890712 cites W2125397084 @default.
• W2065890712 cites W2125894311 @default.
• W2065890712 cites W2137621403 @default.
• W2065890712 cites W2139545593 @default.
• W2065890712 cites W2141166313 @default.
• W2065890712 cites W2145957743 @default.
• W2065890712 cites W2150955509 @default.
• W2065890712 cites W2151916582 @default.
• W2065890712 cites W2156663192 @default.
• W2065890712 cites W2156960076 @default.
• W2065890712 cites W2162578061 @default.
• W2065890712 cites W2162877448 @default.
• W2065890712 cites W2165385329 @default.
• W2065890712 cites W2168352283 @default.
• W2065890712 cites W2169568623 @default.
• W2065890712 cites W2172189554 @default.
• W2065890712 cites W4243629109 @default.
• W2065890712 cites W63076247 @default.
• W2065890712 doi "https://doi.org/10.1016/j.mimet.2005.04.012" @default.
• W2065890712 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15979746" @default.
• W2065890712 hasPublicationYear "2005" @default.
• W2065890712 type Work @default.
• W2065890712 sameAs 2065890712 @default.
• W2065890712 citedByCount "65" @default.
• W2065890712 countsByYear W20658907122012 @default.
• W2065890712 countsByYear W20658907122013 @default.
• W2065890712 countsByYear W20658907122014 @default.
• W2065890712 countsByYear W20658907122015 @default.
• W2065890712 countsByYear W20658907122016 @default.
• W2065890712 countsByYear W20658907122017 @default.
• W2065890712 countsByYear W20658907122020 @default.
• W2065890712 countsByYear W20658907122021 @default.
• W2065890712 countsByYear W20658907122022 @default.
• W2065890712 crossrefType "journal-article" @default.
• W2065890712 hasAuthorship W2065890712A5016193487 @default.
• W2065890712 hasAuthorship W2065890712A5022220145 @default.
• W2065890712 hasAuthorship W2065890712A5037566826 @default.
• W2065890712 hasAuthorship W2065890712A5037897736 @default.
• W2065890712 hasAuthorship W2065890712A5057904055 @default.
• W2065890712 hasAuthorship W2065890712A5086271534 @default.
• W2065890712 hasAuthorship W2065890712A5086936251 @default.
• W2065890712 hasAuthorship W2065890712A5087963967 @default.
• W2065890712 hasConcept C10390740 @default.
• W2065890712 hasConcept C104317684 @default.
• W2065890712 hasConcept C112190416 @default.
• W2065890712 hasConcept C150194340 @default.
• W2065890712 hasConcept C15151743 @default.
• W2065890712 hasConcept C153911025 @default.
• W2065890712 hasConcept C185592680 @default.
• W2065890712 hasConcept C24107716 @default.
• W2065890712 hasConcept C2781053203 @default.
• W2065890712 hasConcept C43617362 @default.
• W2065890712 hasConcept C55493867 @default.
• W2065890712 hasConcept C67705224 @default.
• W2065890712 hasConcept C86803240 @default.
• W2065890712 hasConcept C95371953 @default.
• W2065890712 hasConceptScore W2065890712C10390740 @default.
• W2065890712 hasConceptScore W2065890712C104317684 @default.
• W2065890712 hasConceptScore W2065890712C112190416 @default.
• W2065890712 hasConceptScore W2065890712C150194340 @default.
• W2065890712 hasConceptScore W2065890712C15151743 @default.
• W2065890712 hasConceptScore W2065890712C153911025 @default.
• W2065890712 hasConceptScore W2065890712C185592680 @default.

• next