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• W2065921316 endingPage "8872" @default.
• W2065921316 startingPage "8857" @default.
• W2065921316 abstract "We have identified a novel enzymatic reaction for nitrophorin 2 (NP2), a heme protein previously characterized as a nitric oxide carrier in the saliva of the Rhodnius prolixus insect. NP2 exhibited levels of peroxidase activity comparable to those of the bifunctional peroxidases (KatGs), despite their heme pocket structural differences (heme ruffling, Tyr38 and Tyr85 in hydrogen bonding interactions with the propionates in NP2). The intermediates of the peroxidase-like reaction of NP2 were identified by Electron Paramagnetic Resonance (EPR) and electronic absorption spectroscopies. The EPR spectrum consistent with an [FeIV═O Por•]+ species was detected at pH <7. At pH ≥7, the change from a strong to a weak antiferromagnetic coupling interaction for the [FeIV═O Por•]+ species was accompanied by the subsequent formation of an [FeIV═O Por](Tyr•) intermediate. Tyr38 was shown to be the unique naturally occurring radical site in NP2. The Y38F mutant stabilized the radical on the tyrosine in hydrogen-bonding interaction with the other heme propionate (Tyr85). Kinetic studies using stopped-flow electronic absorption spectrophotometry revealed that the [FeIV═O Por•]+ species reacts with histamine and norepinephrine in a peroxidase-like manner. Our findings demonstrate that NP2 has pH-dependent dual function: at the acidic pH of the insect saliva the protein behaves as a NO carrier, and, if exposed to the higher pH of the tissues and capillaries of the host, NP2 is able to bind histamine or it can efficiently inactivate norepinephrine through a peroxidase-like reaction, in the presence of hydrogen peroxide. Accordingly, the unprecedented peroxidase-like activity of NP2 is concluded to be a key biological function." @default.
• W2065921316 created "2016-06-24" @default.
• W2065921316 creator A5007172461 @default.
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• W2065921316 date "2010-09-23" @default.
• W2065921316 modified "2023-09-25" @default.
• W2065921316 title "Unprecedented Peroxidase-like Activity of <i>Rhodnius prolixus</i> Nitrophorin 2: Identification of the [Fe^IV═O Por^•]⁺ and [Fe^IV═O Por](Tyr38^•) Intermediates and Their Role(s) in Substrate Oxidation" @default.
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• W2065921316 doi "https://doi.org/10.1021/bi100499a" @default.
• W2065921316 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20726527" @default.
• W2065921316 hasPublicationYear "2010" @default.
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