FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2065963898> ?p ?o ?g. }

• W2065963898 endingPage "161" @default.
• W2065963898 startingPage "157" @default.
• W2065963898 abstract "σ receptors represent a potential drug target for numerous therapeutic indications including cancer, depression, psychostimulant abuse, and stroke. Most published radioligand binding studies for σ receptors utilize a low throughput method employing a “cell harvester.” Higher throughput methods are required to facilitate efficient screening of large numbers of novel compounds. In this study, a series of reference compounds was analyzed with a new medium-throughput 96-well filtration method and the results were compared to those obtained using the conventional cell harvester-based method. The 96-well assay utilized rat liver membranes for the determination of both known σ receptor subtypes (σ 1 and σ 2 ) because this tissue contains high densities of both subtypes and fulfills criteria required for reliable use with the 96-well format. The new method gave comparable K i values for reference ligands analyzed in parallel with samples prepared in rat brain membranes and processed on the traditional cell harvester. For σ 1 receptors, equivalent affinity values were observed for both methods/tissues. For σ 2 receptors, approximately 2-fold higher affinities were observed for most compounds in liver, as compared to brain membranes, but excellent correlation with brain-derived values was maintained. To further demonstrate the utility of the new method it was used to screen a novel series of 2(3H)-benzothiazolone compounds, resulting in the identification of several analogues with nanomolar affinity and greater than 50-fold specificity for σ 1 versus σ 2 receptors." @default.
• W2065963898 created "2016-06-24" @default.
• W2065963898 creator A5044827754 @default.
• W2065963898 creator A5070399107 @default.
• W2065963898 creator A5072704108 @default.
• W2065963898 creator A5077693595 @default.
• W2065963898 creator A5087856092 @default.
• W2065963898 date "2012-12-01" @default.
• W2065963898 modified "2023-09-27" @default.
• W2065963898 title "A 96-well filtration method for radioligand binding analysis of σ receptor ligands" @default.
• W2065963898 cites W161356906 @default.
• W2065963898 cites W1970897378 @default.
• W2065963898 cites W1972071899 @default.
• W2065963898 cites W1976929607 @default.
• W2065963898 cites W1979175249 @default.
• W2065963898 cites W1979930380 @default.
• W2065963898 cites W1985444891 @default.
• W2065963898 cites W1992768650 @default.
• W2065963898 cites W1993389380 @default.
• W2065963898 cites W1994153124 @default.
• W2065963898 cites W1994224293 @default.
• W2065963898 cites W2000941955 @default.
• W2065963898 cites W2001369831 @default.
• W2065963898 cites W2001519015 @default.
• W2065963898 cites W2002401947 @default.
• W2065963898 cites W2006283790 @default.
• W2065963898 cites W2011710586 @default.
• W2065963898 cites W2014909377 @default.
• W2065963898 cites W2014956929 @default.
• W2065963898 cites W2021465842 @default.
• W2065963898 cites W2023202438 @default.
• W2065963898 cites W2024026802 @default.
• W2065963898 cites W2025419017 @default.
• W2065963898 cites W2026692772 @default.
• W2065963898 cites W2027105757 @default.
• W2065963898 cites W2031823540 @default.
• W2065963898 cites W2033198571 @default.
• W2065963898 cites W2036195467 @default.
• W2065963898 cites W2040395804 @default.
• W2065963898 cites W2046943917 @default.
• W2065963898 cites W2048719783 @default.
• W2065963898 cites W2050563394 @default.
• W2065963898 cites W2052813372 @default.
• W2065963898 cites W2054597500 @default.
• W2065963898 cites W2055897353 @default.
• W2065963898 cites W2067072499 @default.
• W2065963898 cites W2069783285 @default.
• W2065963898 cites W2070995609 @default.
• W2065963898 cites W2074631079 @default.
• W2065963898 cites W2113924760 @default.
• W2065963898 cites W2158988264 @default.
• W2065963898 cites W2162861469 @default.
• W2065963898 doi "https://doi.org/10.1016/j.jpba.2012.07.023" @default.
• W2065963898 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3726061" @default.
• W2065963898 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22910107" @default.
• W2065963898 hasPublicationYear "2012" @default.
• W2065963898 type Work @default.
• W2065963898 sameAs 2065963898 @default.
• W2065963898 citedByCount "8" @default.
• W2065963898 countsByYear W20659638982013 @default.
• W2065963898 countsByYear W20659638982014 @default.
• W2065963898 countsByYear W20659638982015 @default.
• W2065963898 countsByYear W20659638982016 @default.
• W2065963898 countsByYear W20659638982021 @default.
• W2065963898 crossrefType "journal-article" @default.
• W2065963898 hasAuthorship W2065963898A5044827754 @default.
• W2065963898 hasAuthorship W2065963898A5070399107 @default.
• W2065963898 hasAuthorship W2065963898A5072704108 @default.
• W2065963898 hasAuthorship W2065963898A5077693595 @default.
• W2065963898 hasAuthorship W2065963898A5087856092 @default.
• W2065963898 hasBestOaLocation W20659638982 @default.
• W2065963898 hasConcept C116569031 @default.
• W2065963898 hasConcept C1491633281 @default.
• W2065963898 hasConcept C170493617 @default.
• W2065963898 hasConcept C185592680 @default.
• W2065963898 hasConcept C19052677 @default.
• W2065963898 hasConcept C21951064 @default.
• W2065963898 hasConcept C2909005528 @default.
• W2065963898 hasConcept C41625074 @default.
• W2065963898 hasConcept C43617362 @default.
• W2065963898 hasConcept C55493867 @default.
• W2065963898 hasConcept C67847695 @default.
• W2065963898 hasConcept C85196483 @default.
• W2065963898 hasConceptScore W2065963898C116569031 @default.
• W2065963898 hasConceptScore W2065963898C1491633281 @default.
• W2065963898 hasConceptScore W2065963898C170493617 @default.
• W2065963898 hasConceptScore W2065963898C185592680 @default.
• W2065963898 hasConceptScore W2065963898C19052677 @default.
• W2065963898 hasConceptScore W2065963898C21951064 @default.
• W2065963898 hasConceptScore W2065963898C2909005528 @default.
• W2065963898 hasConceptScore W2065963898C41625074 @default.
• W2065963898 hasConceptScore W2065963898C43617362 @default.
• W2065963898 hasConceptScore W2065963898C55493867 @default.
• W2065963898 hasConceptScore W2065963898C67847695 @default.
• W2065963898 hasConceptScore W2065963898C85196483 @default.
• W2065963898 hasLocation W20659638981 @default.
• W2065963898 hasLocation W20659638982 @default.
• W2065963898 hasLocation W20659638983 @default.

• next