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• W2066011377 startingPage "483" @default.
• W2066011377 abstract "Genomic DNA of Ostreococcus tauri, a fully sequenced marine unicellular alga from the phytoplankton, was used to amplify a gene coding for a typical front-end desaturase involved in polyunsaturated fatty acid biosynthesis. Heterologous expression in Saccharomyces cerevisiae revealed very high desaturation activity with Delta6-regioselectivity. Short-time kinetic experiments showed that the desaturase product was detected in the acyl-CoA pool 5 min after addition of the exogenous substrate to the yeast medium and long before its appearance in the total fatty acids. When this desaturase was co-expressed with the acyl-CoA Delta6-elongase from Physcomitrella patens and the lipid-linked Delta5-desaturase from Phaeodactylum tricornutum, high proportions of arachidonic or eicosapentaenoic acid were obtained, because nearly all of the Delta6-desaturated products were elongated. Furthermore, the product/educt ratios calculated in each glycerolipid for the Delta6-desaturase or for the acyl-CoA Delta6-elongase were in about the same range, whereas this ratio showed a very uneven profile in the case of the lipid-linked Delta5-desaturase. Finally, a sequence-based comparison of all the functionally characterized Delta6-desaturases showed that this enzyme was not related to any previously described sequence. Altogether, our data suggest that this desaturase from O. tauri is an acyl-CoA Delta6-desaturase, the first one cloned from a photosynthetically active organism." @default.
• W2066011377 created "2016-06-24" @default.
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• W2066011377 date "2005-07-05" @default.
• W2066011377 modified "2023-10-01" @default.
• W2066011377 title "<i>In vivo</i> characterization of the first acyl-CoA Δ6-desaturase from a member of the plant kingdom, the microalga <i>Ostreococcus tauri</i>" @default.
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• W2066011377 doi "https://doi.org/10.1042/bj20050111" @default.
• W2066011377 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1175126" @default.
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