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- W2066017424 abstract "Suspension cultures of Catharanthus roseus G. Don cells were grown at various temperatures from 16°C to 40°C. The doubling time of cell populations was strongly increased by a fall in temperature from 27°C to 16°C. The cell cycle parameters of cell suspensions grown at 20°C and 27°C were analysed by the fraction of labelled mitoses method and compared. All cell cycle phases were affected by the shift in temperature with a higher sensitivity of the G1 phase. Doubling times of cell populations and total cell cycle times were about the same at 20°C or 27°C. Consequently under these conditions all cells contributed to the growth of the populations. A strong influence of temperature on the amount per cell of the crude alkaloid fraction or on the specific content of serpentine and ajmalicine was observed. Lowering temperature from 27°C to 16°C resulted in a much higher alkaloid content, conversely when temperature was increased from 27°C to 38°C, the alkaloid content was strongly reduced." @default.
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- W2066017424 date "1980-03-01" @default.
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- W2066017424 title "Temperature response of Catharanthus roseus cells cultivated in liquid medium" @default.
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- W2066017424 doi "https://doi.org/10.1016/0304-4211(80)90135-2" @default.
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