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- W2066060664 abstract "The antitumor activity of alkyl lysophospholipids has raised some questions concerning the degradation ofO-alkyl bonds in naturally occurring ether lipids. In this report, we describe the first continuous assay forO-alkylglycerol monooxygenase (AGMO), the only enzyme known to cleave theO-alkyl bond in saturated ether lipids and ether phospholipids. AGMO activity was monitored at 340 nm by coupling the NADH redox reaction to the tetrahydropteridine cofactor of the rat liver microsomal enzyme. Turnover rates as low as 0.6 nmol/min could be measured. Using radiolabeled substrates, the products were identified with a TLC-Linear-Analyzer. The only interference with this assay can arise from other reducing agents, e.g. dithiothreitol. The assay was used to develop protocols for the solubilization of AGMO from membrane preparations in the presence of detergents." @default.
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- W2066060664 date "1987-11-01" @default.
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- W2066060664 title "A continuous assay forO-alkylglycerol monooxygenase (E.C. 1.14.16.5)" @default.
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- W2066060664 doi "https://doi.org/10.1007/bf02535538" @default.
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