Matches in SemOpenAlex for { <https://semopenalex.org/work/W2066083913> ?p ?o ?g. }
- W2066083913 endingPage "19227" @default.
- W2066083913 startingPage "19220" @default.
- W2066083913 abstract "We characterized three d-galactosyl-beta1-->3-N-acetyl-d-hexosamine phosphorylase (EC 2.4.1.211) homologs from Clostridium phytofermentans (Cphy0577, Cphy1920, and Cphy3030 proteins). Cphy0577 and Cphy3030 proteins exhibited similar activity on galacto-N-biose (GNB; d-Gal-beta1-->3-d-GalNAc) and lacto-N-biose I (LNB; d-Gal-beta1-->3-d-GlcNAc), thus indicating that they are d-galactosyl-beta1-->3-N-acetyl-d-hexosamine phosphorylases, subclassified as GNB/LNB phosphorylase. In contrast, Cphy1920 protein phosphorolyzed neither GNB nor LNB. It showed the highest activity with l-rhamnose as the acceptor in the reverse reaction using alpha-d-galactose 1-phosphate as the donor. The reaction product was d-galactosyl-beta1-->4-l-rhamnose. The enzyme also showed activity on l-mannose, l-lyxose, d-glucose, 2-deoxy-d-glucose, and d-galactose in this order. When d-glucose derivatives were used as acceptors, reaction products were beta-1,3-galactosides. Kinetic parameters of phosphorolytic activity on d-galactosyl-beta1-->4-l-rhamnose were k(cat) = 45 s(-1) and K(m) = 7.9 mm, thus indicating that these values are common among other phosphorylases. We propose d-galactosyl-beta1-->4-l-rhamnose phosphorylase as the name for Cphy1920 protein." @default.
- W2066083913 created "2016-06-24" @default.
- W2066083913 creator A5021258350 @default.
- W2066083913 creator A5041422951 @default.
- W2066083913 creator A5090197610 @default.
- W2066083913 date "2009-07-01" @default.
- W2066083913 modified "2023-09-28" @default.
- W2066083913 title "Characterization of Three β-Galactoside Phosphorylases from Clostridium phytofermentans" @default.
- W2066083913 cites W1516204830 @default.
- W2066083913 cites W1819899307 @default.
- W2066083913 cites W1905297250 @default.
- W2066083913 cites W1933291616 @default.
- W2066083913 cites W1977857958 @default.
- W2066083913 cites W1978529058 @default.
- W2066083913 cites W1982096041 @default.
- W2066083913 cites W1983194790 @default.
- W2066083913 cites W1986746147 @default.
- W2066083913 cites W198768300 @default.
- W2066083913 cites W1987797771 @default.
- W2066083913 cites W1994403203 @default.
- W2066083913 cites W2004948135 @default.
- W2066083913 cites W2005610194 @default.
- W2066083913 cites W2011452259 @default.
- W2066083913 cites W2015600669 @default.
- W2066083913 cites W2024098361 @default.
- W2066083913 cites W2031823104 @default.
- W2066083913 cites W2047354423 @default.
- W2066083913 cites W2049316018 @default.
- W2066083913 cites W2058595703 @default.
- W2066083913 cites W2065280089 @default.
- W2066083913 cites W2071626786 @default.
- W2066083913 cites W2073522850 @default.
- W2066083913 cites W2079386949 @default.
- W2066083913 cites W2081200151 @default.
- W2066083913 cites W2085367624 @default.
- W2066083913 cites W2090975065 @default.
- W2066083913 cites W2099094637 @default.
- W2066083913 cites W2108929776 @default.
- W2066083913 cites W2113964973 @default.
- W2066083913 cites W2129991881 @default.
- W2066083913 cites W2133534580 @default.
- W2066083913 cites W2138462606 @default.
- W2066083913 cites W2152038701 @default.
- W2066083913 cites W2155136534 @default.
- W2066083913 cites W2161746138 @default.
- W2066083913 cites W2165908394 @default.
- W2066083913 cites W2167768477 @default.
- W2066083913 cites W2170904778 @default.
- W2066083913 cites W2171091522 @default.
- W2066083913 cites W2317029767 @default.
- W2066083913 doi "https://doi.org/10.1074/jbc.m109.007666" @default.
- W2066083913 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2740546" @default.
- W2066083913 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19491100" @default.
- W2066083913 hasPublicationYear "2009" @default.
- W2066083913 type Work @default.
- W2066083913 sameAs 2066083913 @default.
- W2066083913 citedByCount "38" @default.
- W2066083913 countsByYear W20660839132012 @default.
- W2066083913 countsByYear W20660839132013 @default.
- W2066083913 countsByYear W20660839132014 @default.
- W2066083913 countsByYear W20660839132015 @default.
- W2066083913 countsByYear W20660839132017 @default.
- W2066083913 countsByYear W20660839132020 @default.
- W2066083913 countsByYear W20660839132021 @default.
- W2066083913 countsByYear W20660839132022 @default.
- W2066083913 crossrefType "journal-article" @default.
- W2066083913 hasAuthorship W2066083913A5021258350 @default.
- W2066083913 hasAuthorship W2066083913A5041422951 @default.
- W2066083913 hasAuthorship W2066083913A5090197610 @default.
- W2066083913 hasBestOaLocation W20660839131 @default.
- W2066083913 hasConcept C181199279 @default.
- W2066083913 hasConcept C185592680 @default.
- W2066083913 hasConcept C204779464 @default.
- W2066083913 hasConcept C2776392439 @default.
- W2066083913 hasConcept C2778173381 @default.
- W2066083913 hasConcept C2779213145 @default.
- W2066083913 hasConcept C55493867 @default.
- W2066083913 hasConcept C71240020 @default.
- W2066083913 hasConceptScore W2066083913C181199279 @default.
- W2066083913 hasConceptScore W2066083913C185592680 @default.
- W2066083913 hasConceptScore W2066083913C204779464 @default.
- W2066083913 hasConceptScore W2066083913C2776392439 @default.
- W2066083913 hasConceptScore W2066083913C2778173381 @default.
- W2066083913 hasConceptScore W2066083913C2779213145 @default.
- W2066083913 hasConceptScore W2066083913C55493867 @default.
- W2066083913 hasConceptScore W2066083913C71240020 @default.
- W2066083913 hasIssue "29" @default.
- W2066083913 hasLocation W20660839131 @default.
- W2066083913 hasLocation W20660839132 @default.
- W2066083913 hasLocation W20660839133 @default.
- W2066083913 hasLocation W20660839134 @default.
- W2066083913 hasOpenAccess W2066083913 @default.
- W2066083913 hasPrimaryLocation W20660839131 @default.
- W2066083913 hasRelatedWork W1978403085 @default.
- W2066083913 hasRelatedWork W2007311407 @default.
- W2066083913 hasRelatedWork W2041074211 @default.
- W2066083913 hasRelatedWork W2051436148 @default.
- W2066083913 hasRelatedWork W2064796459 @default.