FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2066164053> ?p ?o ?g. }

• W2066164053 endingPage "308" @default.
• W2066164053 startingPage "302" @default.
• W2066164053 abstract "The aim of the present study was to purify and characterize classic pancreatic lipase from the reptile turtle (TuPL). Methods: The lipase was purified from the fresh pancreas extract followed by diethylamino ethyl-cellulose chromatography, Sephacryl S-200 gel filtration, and a Mono-Q Sepharose chromatography. Results: Turtle pancreatic lipase is a serine enzyme and it contains only 1 free cysteine. Its activity is maximum at pH 8.2 and 37°C. A specific activity of 10.000 U/mg and 5.000 U/mg were measured titrimetrically on tributyrin and olive oil emulsion, respectively. Natural detergents act as potent inhibitors of TuPL, and colipase restores the activity. When the lipase is inhibited by synthetic detergent, simultaneous addition of colipase and bile salts is required to restore the TuPL activity. The critical surface pressure of TuPL (πc = 20.9 mN m−1) is similar to the one of human PL (πc = 18 mN m−1). Abbreviations: PL - pancreatic lipase, HPL - human pancreatic lipase, PPL - porcine pancreatic lipase, TuPL - turtle pancreatic lipase, NaDC - sodium deoxycholate, NaTDC - sodium taurodeoxycholate, DTNB - 5,5′-dithio-bis-(2-nitrobenzoic acid), PVDF - polyvinylidene difluoride, SDS-PAGE - sodium dodecyl sulfate polyacrylamide gel electrophoresis, TC4 - tributyrin, PC - phosphatidylcholine, THL - tetrahydrolipstatine, IU - international units Conclusions: The results presented in this article indicate that despite the primitive character of the turtle, no significant difference has been observed between TuPL and known mammalian PLs. However, partial proteolysis of TuPL with chymotrypsin shows the absence of the 14-kDa fragment identified as the C-terminal domain in the case of many classic PLs." @default.
• W2066164053 created "2016-06-24" @default.
• W2066164053 creator A5040786260 @default.
• W2066164053 creator A5043400493 @default.
• W2066164053 creator A5073261829 @default.
• W2066164053 date "2008-04-01" @default.
• W2066164053 modified "2023-09-25" @default.
• W2066164053 title "Purification and Characterization of the First Reptile Pancreatic Lipase" @default.
• W2066164053 cites W1558202631 @default.
• W2066164053 cites W1568811076 @default.
• W2066164053 cites W1597793844 @default.
• W2066164053 cites W1600871831 @default.
• W2066164053 cites W1902233018 @default.
• W2066164053 cites W1967911168 @default.
• W2066164053 cites W1968293356 @default.
• W2066164053 cites W1976701629 @default.
• W2066164053 cites W1994373116 @default.
• W2066164053 cites W2004782776 @default.
• W2066164053 cites W2005586803 @default.
• W2066164053 cites W2012176370 @default.
• W2066164053 cites W2015784447 @default.
• W2066164053 cites W2016266875 @default.
• W2066164053 cites W2027300341 @default.
• W2066164053 cites W2027453690 @default.
• W2066164053 cites W2027968658 @default.
• W2066164053 cites W2028606875 @default.
• W2066164053 cites W2030637880 @default.
• W2066164053 cites W2033784547 @default.
• W2066164053 cites W2033989837 @default.
• W2066164053 cites W2057156230 @default.
• W2066164053 cites W2068686352 @default.
• W2066164053 cites W2074203123 @default.
• W2066164053 cites W2074826777 @default.
• W2066164053 cites W2077416030 @default.
• W2066164053 cites W2082015820 @default.
• W2066164053 cites W2082114784 @default.
• W2066164053 cites W2085311652 @default.
• W2066164053 cites W2100837269 @default.
• W2066164053 cites W2414307244 @default.
• W2066164053 cites W4293247451 @default.
• W2066164053 doi "https://doi.org/10.1097/mpa.0b013e31814b9751" @default.
• W2066164053 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18362845" @default.
• W2066164053 hasPublicationYear "2008" @default.
• W2066164053 type Work @default.
• W2066164053 sameAs 2066164053 @default.
• W2066164053 citedByCount "3" @default.
• W2066164053 countsByYear W20661640532016 @default.
• W2066164053 countsByYear W20661640532019 @default.
• W2066164053 countsByYear W20661640532021 @default.
• W2066164053 crossrefType "journal-article" @default.
• W2066164053 hasAuthorship W2066164053A5040786260 @default.
• W2066164053 hasAuthorship W2066164053A5043400493 @default.
• W2066164053 hasAuthorship W2066164053A5073261829 @default.
• W2066164053 hasConcept C101660505 @default.
• W2066164053 hasConcept C127947577 @default.
• W2066164053 hasConcept C181199279 @default.
• W2066164053 hasConcept C185592680 @default.
• W2066164053 hasConcept C193363816 @default.
• W2066164053 hasConcept C2778855143 @default.
• W2066164053 hasConcept C2778913249 @default.
• W2066164053 hasConcept C2779697368 @default.
• W2066164053 hasConcept C2780786110 @default.
• W2066164053 hasConcept C43617362 @default.
• W2066164053 hasConcept C55493867 @default.
• W2066164053 hasConceptScore W2066164053C101660505 @default.
• W2066164053 hasConceptScore W2066164053C127947577 @default.
• W2066164053 hasConceptScore W2066164053C181199279 @default.
• W2066164053 hasConceptScore W2066164053C185592680 @default.
• W2066164053 hasConceptScore W2066164053C193363816 @default.
• W2066164053 hasConceptScore W2066164053C2778855143 @default.
• W2066164053 hasConceptScore W2066164053C2778913249 @default.
• W2066164053 hasConceptScore W2066164053C2779697368 @default.
• W2066164053 hasConceptScore W2066164053C2780786110 @default.
• W2066164053 hasConceptScore W2066164053C43617362 @default.
• W2066164053 hasConceptScore W2066164053C55493867 @default.
• W2066164053 hasIssue "3" @default.
• W2066164053 hasLocation W20661640531 @default.
• W2066164053 hasLocation W20661640532 @default.
• W2066164053 hasOpenAccess W2066164053 @default.
• W2066164053 hasPrimaryLocation W20661640531 @default.
• W2066164053 hasRelatedWork W1991867703 @default.
• W2066164053 hasRelatedWork W2012886705 @default.
• W2066164053 hasRelatedWork W2037938119 @default.
• W2066164053 hasRelatedWork W2040551576 @default.
• W2066164053 hasRelatedWork W2054178137 @default.
• W2066164053 hasRelatedWork W2070685839 @default.
• W2066164053 hasRelatedWork W2091963064 @default.
• W2066164053 hasRelatedWork W2165757187 @default.
• W2066164053 hasRelatedWork W2460422697 @default.
• W2066164053 hasRelatedWork W1562730827 @default.
• W2066164053 hasVolume "36" @default.
• W2066164053 isParatext "false" @default.
• W2066164053 isRetracted "false" @default.
• W2066164053 magId "2066164053" @default.
• W2066164053 workType "article" @default.