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- W2066176838 abstract "To investigate how isoproterenol, a beta-adrenergic agonist, regulates airway ciliary beat frequency (CBF), we simultaneously quantified changes in rabbit airway CBF and intracellular calcium concentration ([Ca2+]i) in response to isoproterenol (100 micromol/L) by using high-speed (240 frames per second) and fluorescence microscopy. At approximately 30 degrees C, isoproterenol induced a change in CBF that could be separated into two parts. First, isoproterenol induced a moderate increase in the basal CBF that was calcium-independent. This response was unaffected by buffering the [Ca2+]i with 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid tetrapotassium salt (BAPTA). Second, isoproterenol induced a transient increase in CBF that was superimposed on the increased basal CBF and correlated with a simultaneous transient increase in [Ca2+]i. This transient increase in CBF was abolished by BAPTA. We conclude that isoproterenol initially increases CBF through a calcium-independent mechanism, probably via protein kinase A, and subsequently through a calcium-dependent mechanism mediated by an increase in [Ca2+]i." @default.
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- W2066176838 date "2005-05-01" @default.
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- W2066176838 title "Effect of Isoproterenol on the Regulation of Rabbit Airway Ciliary Beat Frequency Measured with High-Speed Digital and Fluorescence Microscopy" @default.
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- W2066176838 doi "https://doi.org/10.1177/000348940511400512" @default.
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