Matches in SemOpenAlex for { <https://semopenalex.org/work/W2066186864> ?p ?o ?g. }
Showing items 1 to 92 of
92
with 100 items per page.
- W2066186864 endingPage "8623" @default.
- W2066186864 startingPage "8616" @default.
- W2066186864 abstract "Cell binding assays on antibody arrays permit the rapid immunophenotyping of living cells. The throughput of the analysis, however, is still limited due to our inability to perform parallel and quantitative detection of cells captured on the array. To address this limitation, we employed here an imaging technique based on surface plasmon resonance (SPR). SPR has been frequently used to monitor capture of proteins on antibody microarrays, while few cases were reported for capture of cells. Antibody arrays were prepared through the photopatterning of an alkanethiol monolayer on a gold-evaporated glass plate and the subsequent immobilization of various antibodies onto 4−9 separate spots created by photopatterning. A glass slip was mounted onto the array with a thin spacer to construct a parallel-plate chamber. Leukemia cells were injected into the chamber to conduct a binding assay, while refractive index changes at the vicinity of the array surface were monitored by SPR imaging. We observed that SPR signals were intensified on specific antibody spots but not on nonspecific spots. Confocal laser scanning microscopy revealed that the observed SPR signals were attributed to cell deformations caused by multivalent interactions with immobilized antibody, which effectively elevated the refractive index of a medium phase within an evanescent field. This effect could be suitably utilized to monitor quantitatively cell binding to multiple spots from a heterogeneous cell population." @default.
- W2066186864 created "2016-06-24" @default.
- W2066186864 creator A5013249193 @default.
- W2066186864 creator A5061501587 @default.
- W2066186864 creator A5063762338 @default.
- W2066186864 creator A5087844313 @default.
- W2066186864 creator A5088241337 @default.
- W2066186864 date "2007-10-10" @default.
- W2066186864 modified "2023-10-16" @default.
- W2066186864 title "High-Throughput Immunophenotyping by Surface Plasmon Resonance Imaging" @default.
- W2066186864 cites W1891662809 @default.
- W2066186864 cites W1976600729 @default.
- W2066186864 cites W1983851456 @default.
- W2066186864 cites W2004906401 @default.
- W2066186864 cites W2023383106 @default.
- W2066186864 cites W2032893394 @default.
- W2066186864 cites W2035180858 @default.
- W2066186864 cites W2035783153 @default.
- W2066186864 cites W2037243028 @default.
- W2066186864 cites W2056483443 @default.
- W2066186864 cites W2061429719 @default.
- W2066186864 cites W2093691382 @default.
- W2066186864 cites W2123342482 @default.
- W2066186864 cites W2127504012 @default.
- W2066186864 cites W2143386169 @default.
- W2066186864 cites W2148329395 @default.
- W2066186864 cites W2158048826 @default.
- W2066186864 cites W2283917576 @default.
- W2066186864 cites W4232837145 @default.
- W2066186864 doi "https://doi.org/10.1021/ac071548s" @default.
- W2066186864 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17927142" @default.
- W2066186864 hasPublicationYear "2007" @default.
- W2066186864 type Work @default.
- W2066186864 sameAs 2066186864 @default.
- W2066186864 citedByCount "19" @default.
- W2066186864 countsByYear W20661868642013 @default.
- W2066186864 countsByYear W20661868642015 @default.
- W2066186864 countsByYear W20661868642016 @default.
- W2066186864 countsByYear W20661868642017 @default.
- W2066186864 countsByYear W20661868642018 @default.
- W2066186864 countsByYear W20661868642020 @default.
- W2066186864 countsByYear W20661868642021 @default.
- W2066186864 crossrefType "journal-article" @default.
- W2066186864 hasAuthorship W2066186864A5013249193 @default.
- W2066186864 hasAuthorship W2066186864A5061501587 @default.
- W2066186864 hasAuthorship W2066186864A5063762338 @default.
- W2066186864 hasAuthorship W2066186864A5087844313 @default.
- W2066186864 hasAuthorship W2066186864A5088241337 @default.
- W2066186864 hasConcept C106847996 @default.
- W2066186864 hasConcept C153911025 @default.
- W2066186864 hasConcept C155672457 @default.
- W2066186864 hasConcept C171250308 @default.
- W2066186864 hasConcept C185592680 @default.
- W2066186864 hasConcept C192562407 @default.
- W2066186864 hasConcept C196166836 @default.
- W2066186864 hasConcept C42067758 @default.
- W2066186864 hasConcept C49040817 @default.
- W2066186864 hasConcept C553184892 @default.
- W2066186864 hasConcept C86803240 @default.
- W2066186864 hasConceptScore W2066186864C106847996 @default.
- W2066186864 hasConceptScore W2066186864C153911025 @default.
- W2066186864 hasConceptScore W2066186864C155672457 @default.
- W2066186864 hasConceptScore W2066186864C171250308 @default.
- W2066186864 hasConceptScore W2066186864C185592680 @default.
- W2066186864 hasConceptScore W2066186864C192562407 @default.
- W2066186864 hasConceptScore W2066186864C196166836 @default.
- W2066186864 hasConceptScore W2066186864C42067758 @default.
- W2066186864 hasConceptScore W2066186864C49040817 @default.
- W2066186864 hasConceptScore W2066186864C553184892 @default.
- W2066186864 hasConceptScore W2066186864C86803240 @default.
- W2066186864 hasIssue "22" @default.
- W2066186864 hasLocation W20661868641 @default.
- W2066186864 hasLocation W20661868642 @default.
- W2066186864 hasOpenAccess W2066186864 @default.
- W2066186864 hasPrimaryLocation W20661868641 @default.
- W2066186864 hasRelatedWork W1997508505 @default.
- W2066186864 hasRelatedWork W2018626937 @default.
- W2066186864 hasRelatedWork W2054640622 @default.
- W2066186864 hasRelatedWork W2055526460 @default.
- W2066186864 hasRelatedWork W2062764377 @default.
- W2066186864 hasRelatedWork W2359322268 @default.
- W2066186864 hasRelatedWork W2368321596 @default.
- W2066186864 hasRelatedWork W2372433843 @default.
- W2066186864 hasRelatedWork W2461073212 @default.
- W2066186864 hasRelatedWork W2911832213 @default.
- W2066186864 hasVolume "79" @default.
- W2066186864 isParatext "false" @default.
- W2066186864 isRetracted "false" @default.
- W2066186864 magId "2066186864" @default.
- W2066186864 workType "article" @default.