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• W2066204038 startingPage "193701" @default.
• W2066204038 abstract "A phase mask design that we term a “bisected pupil” (BSP) provides several advantages for single-molecule optical imaging. When using the BSP with a dual-polarization optical Fourier processing system, both the position and dipole orientation of individual fluorescent molecules may be measured from a single camera image. In the context of single-molecule super-resolution microscopy, this technique permits one to diagnose, and subsequently to remove imaging artifacts resulting from orientation-induced localization errors. If the molecules labeling a structure are rotationally mobile, thus mitigating dipole orientation errors, this technique enables super-resolution imaging in three dimensions. We present simulations and experimental verification." @default.
• W2066204038 created "2016-06-24" @default.
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• W2066204038 date "2014-05-12" @default.
• W2066204038 modified "2023-10-18" @default.
• W2066204038 title "A bisected pupil for studying single-molecule orientational dynamics and its application to three-dimensional super-resolution microscopy" @default.
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• W2066204038 doi "https://doi.org/10.1063/1.4876440" @default.
• W2066204038 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4032398" @default.
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