Matches in SemOpenAlex for { <https://semopenalex.org/work/W2066274993> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W2066274993 endingPage "222" @default.
- W2066274993 startingPage "211" @default.
- W2066274993 abstract "Ca influx in cultured neonatal myocardium can be augmented by metabolic inhibition or free radical exposure. This increase cannot be prevented by blockade of L-type Ca channels or inhibition of Na-Ca exchange. It is speculated that a specific Ca leak may be involved in this process. In the present study patch clamp techniques were used to examine this hypothesis. Currents were measured in a recording configuration of cell attached or excised inside-out membrane patches in adult rat ventricular myocytes as affected by metabolic inhibition or free radical exposure. The metabolic inhibition was achieved by use of 1 mM iodoacetic acid and 10 mM 2-deoxyglucose with omission of glucose in the perfusate. Free radicals were generated by application of 100 microM hydrogen peroxide in the perfusate. Specific Ca leak channels were identified. The channels were not significantly permeable to monovalent cations. The activity of these channels was increased markedly over a period of minutes by metabolic inhibition or free radical exposure. In the presence of 100 microM hydrogen peroxide the open probability (NPo) of the channels increased from 0.0083 +/- 0.003 (mean +/- S.D.) in control to 0.09 +/- 0.024 (P < 0.01). During metabolic inhibition it was augmented from 0.0075 +/- 0.0035 to 0.062 +/- 0.018 (P < 0.01). The increase of the Ca leak channel activity under both conditions could be completely blocked by addition of polycationic protamine to the patch pipette solution. The results support the hypothesis that Ca leak channels are involved in the Ca overload induced by metabolic inhibition or free radical exposure. The inhibitory effect of polycation may have important therapeutic implications for the treatment of ischemic cardiac heart disease." @default.
- W2066274993 created "2016-06-24" @default.
- W2066274993 creator A5046453924 @default.
- W2066274993 creator A5066583553 @default.
- W2066274993 creator A5071363385 @default.
- W2066274993 date "1995-01-01" @default.
- W2066274993 modified "2023-09-27" @default.
- W2066274993 title "Increase in calcium leak channel activity by metabolic inhibition or hydrogen peroxide in rat ventricular myocytes and its inhibition by polycation" @default.
- W2066274993 cites W1556814137 @default.
- W2066274993 cites W1901865848 @default.
- W2066274993 cites W1963210861 @default.
- W2066274993 cites W1964978478 @default.
- W2066274993 cites W1973388548 @default.
- W2066274993 cites W1974180042 @default.
- W2066274993 cites W1979966267 @default.
- W2066274993 cites W1983536345 @default.
- W2066274993 cites W1985396526 @default.
- W2066274993 cites W1990705483 @default.
- W2066274993 cites W1999107160 @default.
- W2066274993 cites W2009304088 @default.
- W2066274993 cites W2009667219 @default.
- W2066274993 cites W2009935511 @default.
- W2066274993 cites W2010762668 @default.
- W2066274993 cites W2013247387 @default.
- W2066274993 cites W2033594768 @default.
- W2066274993 cites W2056937880 @default.
- W2066274993 cites W2080669215 @default.
- W2066274993 cites W2094557223 @default.
- W2066274993 cites W2110648972 @default.
- W2066274993 cites W2146447665 @default.
- W2066274993 cites W2146610807 @default.
- W2066274993 cites W2170679542 @default.
- W2066274993 cites W2179663913 @default.
- W2066274993 cites W2185501969 @default.
- W2066274993 cites W2280577476 @default.
- W2066274993 cites W2319662829 @default.
- W2066274993 cites W2338955302 @default.
- W2066274993 cites W2418942336 @default.
- W2066274993 cites W2970948582 @default.
- W2066274993 doi "https://doi.org/10.1016/s0022-2828(08)80020-x" @default.
- W2066274993 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7760345" @default.
- W2066274993 hasPublicationYear "1995" @default.
- W2066274993 type Work @default.
- W2066274993 sameAs 2066274993 @default.
- W2066274993 citedByCount "37" @default.
- W2066274993 countsByYear W20662749932021 @default.
- W2066274993 crossrefType "journal-article" @default.
- W2066274993 hasAuthorship W2066274993A5046453924 @default.
- W2066274993 hasAuthorship W2066274993A5066583553 @default.
- W2066274993 hasAuthorship W2066274993A5071363385 @default.
- W2066274993 hasConcept C12554922 @default.
- W2066274993 hasConcept C134018914 @default.
- W2066274993 hasConcept C139066938 @default.
- W2066274993 hasConcept C147944092 @default.
- W2066274993 hasConcept C170493617 @default.
- W2066274993 hasConcept C178790620 @default.
- W2066274993 hasConcept C181199279 @default.
- W2066274993 hasConcept C185592680 @default.
- W2066274993 hasConcept C207200792 @default.
- W2066274993 hasConcept C2780197888 @default.
- W2066274993 hasConcept C519063684 @default.
- W2066274993 hasConcept C533411734 @default.
- W2066274993 hasConcept C55493867 @default.
- W2066274993 hasConcept C86803240 @default.
- W2066274993 hasConceptScore W2066274993C12554922 @default.
- W2066274993 hasConceptScore W2066274993C134018914 @default.
- W2066274993 hasConceptScore W2066274993C139066938 @default.
- W2066274993 hasConceptScore W2066274993C147944092 @default.
- W2066274993 hasConceptScore W2066274993C170493617 @default.
- W2066274993 hasConceptScore W2066274993C178790620 @default.
- W2066274993 hasConceptScore W2066274993C181199279 @default.
- W2066274993 hasConceptScore W2066274993C185592680 @default.
- W2066274993 hasConceptScore W2066274993C207200792 @default.
- W2066274993 hasConceptScore W2066274993C2780197888 @default.
- W2066274993 hasConceptScore W2066274993C519063684 @default.
- W2066274993 hasConceptScore W2066274993C533411734 @default.
- W2066274993 hasConceptScore W2066274993C55493867 @default.
- W2066274993 hasConceptScore W2066274993C86803240 @default.
- W2066274993 hasIssue "1" @default.
- W2066274993 hasLocation W20662749931 @default.
- W2066274993 hasLocation W20662749932 @default.
- W2066274993 hasOpenAccess W2066274993 @default.
- W2066274993 hasPrimaryLocation W20662749931 @default.
- W2066274993 hasRelatedWork W146230617 @default.
- W2066274993 hasRelatedWork W1969404129 @default.
- W2066274993 hasRelatedWork W2003687245 @default.
- W2066274993 hasRelatedWork W2016661827 @default.
- W2066274993 hasRelatedWork W2153614559 @default.
- W2066274993 hasRelatedWork W2366213467 @default.
- W2066274993 hasRelatedWork W2408635698 @default.
- W2066274993 hasRelatedWork W2411738142 @default.
- W2066274993 hasRelatedWork W2886653979 @default.
- W2066274993 hasRelatedWork W3151887034 @default.
- W2066274993 hasVolume "27" @default.
- W2066274993 isParatext "false" @default.
- W2066274993 isRetracted "false" @default.
- W2066274993 magId "2066274993" @default.
- W2066274993 workType "article" @default.