Matches in SemOpenAlex for { <https://semopenalex.org/work/W2066380513> ?p ?o ?g. }
Showing items 1 to 93 of
93
with 100 items per page.
- W2066380513 endingPage "12" @default.
- W2066380513 startingPage "9" @default.
- W2066380513 abstract "Previous studies have demonstrated that components of the extracellular matrix can induce neurite extension and cell adhesion in the neuroblastoma x glioma hybrid cell line, NG108-15. Using standard intracellular recording techniques, we examined the resting membrane potential (RMP) and membrane excitability of NG108-15 cells differentiated under serum-free media with representative extracellular matrix (ECM) protein components as the substrate. Surfaces coated with collagen IV and a laminin-1 synthetic peptide induced a significantly (P < 0.05) more hyperpolarized RMP than control polystyrene surfaces. For example, after > or =8 days in culture NG108-15 cells plated on polystyrene exhibited a RMP of -33.2+/-0.8 mV (mean+/-SEM, n=158 cells) whereas cells cultured on the laminin-1 peptide C16 and collagen IV showed a RMP of -37.6+/-0.7 mV (n=157) and -37.5+/-1.5 mV (n=68), respectively. Furthermore, the proportions of cells on ECM substrates showing membrane excitability, i.e. evoked action potentials (APs) and the capability for regular firing, were significantly greater compared to those cells cultured on polystyrene. Among excitable cells cultured on the different substrates, characteristics of the action potentials, such as AP duration, amplitude, and the maximum rate of rise, dV/dtMAX, were examined in detail. While little or no differences were observed between polystyrene and the laminin-1 peptide groups, significant differences in the AP parameters were apparent for collagen IV. For example, dV/dtMAX for polystyrene and the laminin-1 peptide C16 were only 71.7+/-24.5 V/s (n=11) and 59.0+/-8.9 V/s (n=9), respectively, whereas cells cultured on collagen IV surfaces exhibited a dV/dtMAX reaching 156.1+/-22.0 V/s (n=7). These data support a role for ECM components in the maintenance of the RMP and membrane excitability in NG108-15 cells." @default.
- W2066380513 created "2016-06-24" @default.
- W2066380513 creator A5007738452 @default.
- W2066380513 creator A5077288891 @default.
- W2066380513 creator A5088790462 @default.
- W2066380513 creator A5090162563 @default.
- W2066380513 date "1998-04-01" @default.
- W2066380513 modified "2023-10-15" @default.
- W2066380513 title "Influence of extracellular matrix proteins on membrane potentials and excitability in NG108-15 cells" @default.
- W2066380513 cites W2009190044 @default.
- W2066380513 cites W2011761081 @default.
- W2066380513 cites W2016840017 @default.
- W2066380513 cites W2037863585 @default.
- W2066380513 cites W2049855417 @default.
- W2066380513 cites W2054907799 @default.
- W2066380513 cites W2063551829 @default.
- W2066380513 cites W2066136725 @default.
- W2066380513 cites W2070252524 @default.
- W2066380513 cites W2071806319 @default.
- W2066380513 cites W2081452775 @default.
- W2066380513 cites W2091726747 @default.
- W2066380513 cites W2132159602 @default.
- W2066380513 cites W2151230921 @default.
- W2066380513 cites W2797855141 @default.
- W2066380513 cites W4210435283 @default.
- W2066380513 cites W1977185894 @default.
- W2066380513 doi "https://doi.org/10.1016/s0304-3940(98)00193-1" @default.
- W2066380513 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9622195" @default.
- W2066380513 hasPublicationYear "1998" @default.
- W2066380513 type Work @default.
- W2066380513 sameAs 2066380513 @default.
- W2066380513 citedByCount "9" @default.
- W2066380513 countsByYear W20663805132012 @default.
- W2066380513 countsByYear W20663805132013 @default.
- W2066380513 countsByYear W20663805132014 @default.
- W2066380513 countsByYear W20663805132022 @default.
- W2066380513 crossrefType "journal-article" @default.
- W2066380513 hasAuthorship W2066380513A5007738452 @default.
- W2066380513 hasAuthorship W2066380513A5077288891 @default.
- W2066380513 hasAuthorship W2066380513A5088790462 @default.
- W2066380513 hasAuthorship W2066380513A5090162563 @default.
- W2066380513 hasConcept C12554922 @default.
- W2066380513 hasConcept C178790620 @default.
- W2066380513 hasConcept C181911157 @default.
- W2066380513 hasConcept C185592680 @default.
- W2066380513 hasConcept C189165786 @default.
- W2066380513 hasConcept C2779281246 @default.
- W2066380513 hasConcept C2779814568 @default.
- W2066380513 hasConcept C28406088 @default.
- W2066380513 hasConcept C41625074 @default.
- W2066380513 hasConcept C54355233 @default.
- W2066380513 hasConcept C55493867 @default.
- W2066380513 hasConcept C79879829 @default.
- W2066380513 hasConcept C81885089 @default.
- W2066380513 hasConcept C84416704 @default.
- W2066380513 hasConcept C86803240 @default.
- W2066380513 hasConceptScore W2066380513C12554922 @default.
- W2066380513 hasConceptScore W2066380513C178790620 @default.
- W2066380513 hasConceptScore W2066380513C181911157 @default.
- W2066380513 hasConceptScore W2066380513C185592680 @default.
- W2066380513 hasConceptScore W2066380513C189165786 @default.
- W2066380513 hasConceptScore W2066380513C2779281246 @default.
- W2066380513 hasConceptScore W2066380513C2779814568 @default.
- W2066380513 hasConceptScore W2066380513C28406088 @default.
- W2066380513 hasConceptScore W2066380513C41625074 @default.
- W2066380513 hasConceptScore W2066380513C54355233 @default.
- W2066380513 hasConceptScore W2066380513C55493867 @default.
- W2066380513 hasConceptScore W2066380513C79879829 @default.
- W2066380513 hasConceptScore W2066380513C81885089 @default.
- W2066380513 hasConceptScore W2066380513C84416704 @default.
- W2066380513 hasConceptScore W2066380513C86803240 @default.
- W2066380513 hasIssue "1" @default.
- W2066380513 hasLocation W20663805131 @default.
- W2066380513 hasLocation W20663805132 @default.
- W2066380513 hasOpenAccess W2066380513 @default.
- W2066380513 hasPrimaryLocation W20663805131 @default.
- W2066380513 hasRelatedWork W1971846421 @default.
- W2066380513 hasRelatedWork W1979456462 @default.
- W2066380513 hasRelatedWork W1982145729 @default.
- W2066380513 hasRelatedWork W1990712257 @default.
- W2066380513 hasRelatedWork W2061840290 @default.
- W2066380513 hasRelatedWork W2063672911 @default.
- W2066380513 hasRelatedWork W2065128345 @default.
- W2066380513 hasRelatedWork W2067330261 @default.
- W2066380513 hasRelatedWork W2108295622 @default.
- W2066380513 hasRelatedWork W190756422 @default.
- W2066380513 hasVolume "246" @default.
- W2066380513 isParatext "false" @default.
- W2066380513 isRetracted "false" @default.
- W2066380513 magId "2066380513" @default.
- W2066380513 workType "article" @default.