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• W2066389610 abstract "The work presented here provides the first intensive insight into the bacterial populations in the digestive tract of the North American moose (Alces alces). Eight free-range moose on natural pasture were sampled, producing eight rumen samples and six colon samples. Second generation (G2) PhyloChips were used to determine the presence of hundreds of operational taxonomic units (OTUs), representing multiple closely related species/strains (>97% identity), found in the rumen and colon of the moose. A total of 789 unique OTUs were used for analysis, which passed the fluorescence and the positive fraction thresholds. There were 73 OTUs, representing 21 bacterial families, which were found exclusively in the rumen samples: Lachnospiraceae, Prevotellaceae and several unclassified families, whereas there were 71 OTUs, representing 22 bacterial families, which were found exclusively in the colon samples: Clostridiaceae, Enterobacteriaceae and several unclassified families. Overall, there were 164 OTUs that were found in 100% of the samples. The Firmicutes were the most dominant bacteria phylum in both the rumen and the colon. Microarray data available at ArrayExpress, accession number E-MEXP-3721. Using PhyloTrac and UniFrac computer software, samples clustered into two distinct groups: rumen and colon, confirming that the rumen and colon are distinct environments. There was an apparent correlation of age to cluster, which will be validated by a larger sample size in future studies, but there were no detectable trends based upon gender." @default.
• W2066389610 created "2016-06-24" @default.
• W2066389610 creator A5032613671 @default.
• W2066389610 creator A5059239350 @default.
• W2066389610 date "2012-01-01" @default.
• W2066389610 modified "2023-10-16" @default.
• W2066389610 title "Insight into the bacterial gut microbiome of the North American moose (Alces alces)" @default.
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• W2066389610 doi "https://doi.org/10.1186/1471-2180-12-212" @default.
• W2066389610 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3585231" @default.
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